利用抑制性削减杂交技术研究抗CD3、CD28单抗共刺激人T淋巴细胞的基因差异表达

发布时间：2018-01-10 21:19

本文关键词：利用抑制性削减杂交技术研究抗CD3、CD28单抗共刺激人T淋巴细胞的基因差异表达　出处：《苏州大学》2006年硕士论文　论文类型：学位论文

【摘要】： 通过TCR产生的第一信号和B7与CD28结合所介导的第二信号,导致人初始T细胞的活化、增殖与免疫反应。这些信号受一个复杂的网络调节,该网络是由许多基因组成和参与的。为了研究参与人T细胞信号转导的基因,本文以正常人初始T细胞作为对照组,以抗人CD3单抗联合抗人CD28单抗激发的人T细胞作为实验组,进行抑制性削减杂交。结果筛选到43个侯选克隆,对其单向测序后,经过与GenBank同源序列比较,证实了4个克隆所编码的基因参与了T细胞信号转导的下游通路,分别为CCND2、RHOF、RHOA和IRF4;新发现19个克隆所代表的基因(其中4个为重复克隆)可能与T细胞信号转导途径有关;另外20个克隆在GenBank中无法查到对应的同源基因,可能代表了新基因。对这些基因的进一步研究,有助于阐释T细胞的信号转导机制,为了解体内活化的T细胞参与的生理和病理过程,具有重要的指导意义。
[Abstract]:The first signal produced by TCR and the second signal mediated by the binding of B7 to CD28 lead to the activation, proliferation and immune response of human initial T cells. These signals are regulated by a complex network. This network is composed of many genes. In order to study the genes involved in T cell signal transduction, we use the normal human initial T cells as the control group. Human T cells stimulated by anti-human CD3 monoclonal antibody and anti-human CD28 monoclonal antibody were used as experimental group to perform suppression subtractive hybridization. Results 43 candidate clones were screened and sequenced. Compared with the homologous sequence of GenBank, it was confirmed that the genes encoded by the four clones were involved in the downstream pathway of T cell signal transduction, namely CCND2RHOFFHOA and IRF4, respectively. The genes represented by 19 clones (4 of which were repeated clones) may be related to the signal transduction pathway of T cells. The other 20 clones could not find the corresponding homologous genes in GenBank, which may represent new genes. Further study of these genes will help to explain the signal transduction mechanism of T cells. In order to understand the physiological and pathological process of activated T cells in vivo, it has important guiding significance.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392;Q789

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