冲击波诱导骨髓间充质干细胞向成骨细胞分化的研究

发布时间：2018-01-10 23:06

本文关键词：冲击波诱导骨髓间充质干细胞向成骨细胞分化的研究　出处：《吉林大学》2007年硕士论文　论文类型：学位论文

【摘要】： 实验目的: 观察在体外培养条件下冲击波诱导健康成年人骨髓间充质干细胞(hMSCs)向成骨细胞分化特点,研究适宜冲击波作用强度,并探讨其机制。实验方法: 1、在体外对健康成年人的骨髓间充质干细胞进行分离、传代培养,经免疫组织化学方法鉴定后,分别采用不同强度的低能冲击波进行诱导,观察细胞变化并进行检测,确定最佳冲击波诱导强度。 2、对鉴定后的骨髓间充质干细胞,采用最佳强度的冲击波进行诱导,并在不同的时间进行RT-PCR检测,观察c-fos和c-jun基因表达的变化。实验结果: 冲击波诱导骨髓间充质干细胞向成骨细胞分化的最佳作用强度为8.5kV,120次,成骨作用明显优于对照组(P0.01)。采用最佳强度冲击波作用后,c-fos和c-jun基因表达量较对照组明显增加,45min达到高峰。实验结论: 低能冲击波在适宜条件下可诱导骨髓间充质干细胞向成骨细胞分化,c-fos和c-jun基因表达增加。
[Abstract]:Objective: Objective: to observe the differentiation of bone marrow mesenchymal stem cells (hMSCs) into osteoblasts induced by shock wave in vitro, to study the suitable shock wave intensity, and to explore its mechanism. Experimental methods: 1. Bone marrow mesenchymal stem cells from healthy adults were isolated and cultured in vitro. The changes of cells were observed and detected to determine the optimal intensity of shock wave induction. 2. The bone marrow mesenchymal stem cells were induced by shock wave with the best intensity, and RT-PCR was detected at different time. The changes of c-fos and c-jun gene expression were observed. Experimental results: The best effect of shock wave on differentiation of bone marrow mesenchymal stem cells into osteoblasts was 8.5 kV / 120 times. The expression of c-fos and c-jun genes was significantly increased to a peak at 45 minutes after the best shock wave was used in the osteogenesis group, and the expression of c-fos and c-jun genes in the control group was significantly higher than that in the control group. The experimental conclusions are as follows: Low energy shock wave could induce the expression of c-fos and c-jun genes in osteoblast differentiation of bone marrow mesenchymal stem cells under suitable conditions.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R329

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