肠免疫系统和HIV感染相关免疫学的研究

发布时间：2018-01-11 00:18

本文关键词：肠免疫系统和HIV感染相关免疫学的研究　出处：《暨南大学》2006年硕士论文　论文类型：学位论文

【摘要】：本研究旨在探讨肠免疫系统有别于系统免疫系统的特征，在此基础上探讨肠相关淋巴组织中淋巴细胞对HIV感染的容许性和HIV复制的支持性，最后，探讨AT-2灭活HIV病毒颗粒在HIV感染时所引起的免疫过度活化的分子基础，为艾滋病以肠道为靶向的新治疗策略的制定奠定免疫学基础。也就是说，通过此研究初步验证我们所提出的“HIV感染肠道免疫系统过度活化论”工作假说。我们的假说认为：在正常情况下，肠道免疫系统处于生理性活化状态和生理性低反应性状态，其生理性活化状态为HIV感染提供了大量的易感细胞。而在HIV感染时，由于危险信号的释放，病毒子模拟APc的作用，调节性T细胞优先被删除等原因导致生理性低反应性状态被打破，而出现免疫过度活化，从而产生更多的HIV易感细胞，通过直接感染以及活化诱导细胞凋亡，使肠道T细胞在短期内大量丢失，，从而导致免疫缺陷。为此，我们进行了三个方面的研究：小鼠肠粘膜免疫系统特点分析；人肠道HIV感染容许性和HIV复制支持性分析；HIV感染免疫过度活化机理体外实验分析。论文的第一部分，通过对小鼠派氏集合淋巴结(PPs)、肠系膜淋巴结(MLNs)和腹股沟淋巴结(ILNs)的比较性研究，以分析小鼠肠道免疫系统的特点。以往的研究表明，由于肠道是机体营养吸收和共生菌繁殖的主要场所，精确区分入侵病原微生物和无害的食物和共生菌抗原成为肠粘膜免疫系统的结构和功能进化的原动力。在功能上，肠道免疫系统能十分清楚地区分哪些是有害的抗原(例如入侵微生物)，哪些是无害的抗原(例如食物抗原和肠道共生菌)，以便作出合适的免疫应答，以清除有害的抗原而对无害的抗原形成耐受，以保护机体免受病理性损伤；在结构上，分为免疫应答“诱导”部位(“inductive”sites)和免疫应答“效应者”部位(“effector”sites)，前者与免疫应答的启动和免疫耐受的诱导有关，后者参与实施免疫应答的效应者机制。本部分我们采取了以下的研究方法：无菌分离小鼠PPs、
[Abstract]:The purpose of this study is to investigate the intestinal immune system has characteristic different from the immune system, on the basis of lymphocytes of gut associated lymphoid tissue to allow and support HIV replication, HIV infection, finally, to explore the molecular basis of AT-2 inactivated HIV virus particles in the disease caused by HIV infection without excessive activation, for to lay the basic immunology AIDS intestinal develop new therapeutic strategies to target. That is to say, through this study preliminary verification of HIV infection of intestinal immune system we proposed "excessive activation of working hypothesis. Our hypothesis is that: under normal circumstances, the intestinal immune system is in the state of physiological activation and physiological low the reaction of the state, the state of physiological activation provides a large number of susceptible cells for HIV infection. In HIV infection, due to the release of danger signals, adenovirus mimic the role of APc, adjusted T Causes of cell priority is deleted as a result of physiological reactivity is broken, and the emergence of immune activation, resulting in more HIV susceptible cells by direct infection and activation induced cell apoptosis, the intestinal T cell loss in the short term, which leads to immune deficiency. Therefore, we conducted a study of three the analysis of the characteristics of intestinal mucosal immune system in mice; human intestinal HIV infection and the supportiveness of HIV replication of HIV infection; immune activation mechanism analysis in vitro.
The first part of the thesis, through the collection of inflammatory bowel disease lymph node (PPs), mesenteric lymph node (MLNs) and inguinal lymph node (ILNs) of the comparative study, to analysis the characteristics of intestinal immune system in mice. Previous studies showed that, due to intestinal absorption of nutrients is the main place of symbiotic bacteria breeding body, accurate to distinguish the invasion of pathogenic microorganisms and harmless commensal bacteria become food and antigen motive power structure and function of intestinal mucosal immune system evolution. In function, the intestinal immune system can clearly distinguish what is harmful antigens (e.g., which is invading microbes) harmless antigens (e.g. food antigens and commensal bacteria) and in order to make the appropriate immune response, to remove harmful antigen and harmless to antigen tolerance, to protect the body from injury; in the structure, divided into "induced" (part of the immune response" Inductive "sites") and the immune response effect "site (" effector "sites), and the former was related to the induction of immune response priming and immune tolerance, which is involved in the implementation of the effect of the immune response mechanism. In this part, we adopt the following methods: sterile from mouse PPs,

【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

【相似文献】