阴道毛滴虫氢化酶体腺苷酸激酶基因的克

发布时间：2018-01-11 15:41

本文关键词：阴道毛滴虫氢化酶体腺苷酸激酶基因的克隆、表达及其种系进化的研究　出处：《四川大学》2006年硕士论文　论文类型：学位论文

【摘要】：研究背景及目的：阴道毛滴虫(Trichomonas vaginalis)是寄生在人体泌尿生殖道的一种常见病原体，，可引起阴道毛滴虫病(Trichomoniasis)。阴道滴虫病是常见的性传播疾病，世界每年约有1亿2千万患者。我国近年来男性及女性感染率均有上升趋势，分别约占性疾病患者的1／4。它是威胁人类健康的主要的性传播疾病之一。腺苷酸激酶(adenylate kinase，简称AK)是细胞中一种重要的核苷酸激酶。腺苷酸激酶以及其它磷酸转移酶对维持细胞中腺苷酸的正常含量及在能量代谢和酶活性调节中起重要作用。为了对付生存环境中激烈、复杂的竞争，需要具有高度活性的AK。它能使阴道毛滴虫顺利完成整个能量代谢过程，从而完成运动、寄生等过程。AK几乎无处不在，像真菌、叶绿体、真核细胞的胞质、内膜和线粒体基质中，它还存在于某些特别的原生生物所具有的膜结合细胞器中，比如毛滴虫的氢化酶体中。到目前为止，已有二十多种生物中的核苷酸激酶的高级结构被破译出来。按照蛋白质大小的不同，这个酶的大家族可划分为两个亚家族，即大核苷酸激酶和小核苷酸激酶，大的一类酶大于24KD，而小的一类酶在21KD左右。这种差异来自于羧基未端的一条长链，它在大的核苷酸激酶中存在，而小的酶类则无。从脊椎动物和酵母菌的研究中发现，这些酶具有细胞中不同位置存在的同工酶形式。在脊椎动物细胞中小的同工酶——AK1是在胞质中存在，而两种较大的AK2、AK3存在于内膜中；有关生物体的种系发育资料表明，AK的演化与生物体中多数亚家族的进化是同步的，提示这种酶与包含它的细胞器的早期分化和演变是同时
[Abstract]:Background and objective: Trichomonas vaginalis is a common pathogen in human genitourinary tract. Trichomoniasis can cause trichomoniasis. Trichomonas vaginalis is a common sexually transmitted disease. There are about 120 million patients in the world every year. In recent years, the infection rate of both males and females in China is on the rise. It is one of the major sexually transmitted diseases that threaten human health. Adenylate kinase. Aka). Adenylate kinase and other phosphotransferases play an important role in maintaining the normal content of adenylate and regulating energy metabolism and enzyme activity. It's intense. Complex competition requires highly active AK. It enables Trichomonas vaginalis to complete the entire energy metabolism process, thus completing the movement, parasitism and other processes almost everywhere, such as fungi, chloroplasts. In the cytoplasm, intima, and mitochondrial matrix of eukaryotic cells, it is also present in the membrane-binding organelles of some particular protozoa, such as the hydrogenated enzyme of Trichomonas trichomonas. The advanced structures of nucleotide kinases in more than 20 organisms have been decoded. According to the size of the protein, the large family of the enzyme can be divided into two subfamilies, large nucleotide kinase and small nucleotide kinase. The large group of enzymes is larger than 24 KD, while the small group of enzymes is about 21 KD. This difference is due to a long chain of carboxyl unterminated, which is present in large nucleotide kinase. Small enzymes are not found in vertebrates and yeasts. In vertebrate cells, the small isozyme AK1 is present in the cytoplasm, while the two larger AK2AK 3 exist in the intima. The phylogenetic data of related organisms indicate that the evolution of AK is synchronized with that of most subfamilies of the organism, suggesting that the enzyme is at the same time as the early differentiation and evolution of the organelles that contain it.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R383

【相似文献】