去氧胆酸钠、2，6-二氯苯腈和LHRH-PE40毒性的动物实验研究

发布时间：2018-01-11 20:15

本文关键词：去氧胆酸钠、2，6-二氯苯腈和LHRH-PE40毒性的动物实验研究　出处：《汕头大学》2006年博士论文　论文类型：学位论文

【摘要】：目的研究去氧胆酸钠和2，6-二氯苯腈局部或全身给药对鼻粘膜的影响，建立药物对鼻腔形态和功能影响的评价方法。方法 1．去氧胆酸钠对wistar大鼠鼻粘膜的影响：(1) 分离大鼠鼻呼吸部粘膜，获得离体呼吸部粘膜纤毛模型，分别置于浓度为0.0001g／mL、0.001g／mL和0.01g／mL的去氧胆酸钠溶液中，显微镜观察并测定纤毛的摆动频率。(2) 采用滴鼻法于大鼠两侧鼻腔同时滴入浓度为0.01g／mL的去氧胆酸钠，每侧10μL，每天1次，连续7天，末次给药2小时后分离出鼻呼吸部粘膜，显微镜观察并测定纤毛摆动频率；末次给药24小时后取鼻，脱钙处理、HE染色，，光镜观察。2．去氧胆酸钠对beagle犬鼻粘膜的影响：采用喷雾法于犬两侧鼻腔同时喷入浓度为0.01g／mL的去氧胆酸钠，每侧100μL，每天1次，连续7天，末次给药2小时后，分别用印度墨汁和核素标记的白蛋白为示踪剂，测定鼻呼吸部粘膜纤毛的输送速度；末次给药24小时后分离鼻呼吸部粘膜，常规制片，扫描电镜和透射电镜观察。3.2，6-二氯苯腈对wistar大鼠鼻粘膜的影响：配制浓度为0.04g／mL的2，6-二氯苯腈溶液，经两种途径分别给药，每天1次，连续7天。其中腹腔给药，每次1mL／kg体重；双侧鼻孔滴鼻给药，每次每侧10μL。光镜、透射电
[Abstract]:Objective To study the effects of sodium deoxycholate and 2,6 - dichlorobenzonitrile topically or systemically on nasal mucosa and to establish a method of evaluating the effect of drugs on nasal morphology and function . The effects of sodium deoxycholate on the nasal mucosa of wistar rats were studied : ( 1 ) To separate the nasal mucosa of rat nasal respiratory tract , and to obtain the isolated respiratory tract mucosa ciliary model , which was placed in the sodium deoxycholate solution with the concentration of 0.0001g / mL , 0.001g / mL and 0.01g / mL , respectively . The effect of sodium deoxycholate on the nasal mucosa of dogs in dogs was studied by spraying sodium deoxycholate at the concentration of 0.01 g / mL on both sides of the nasal cavity at the same time . After the last administration for 24 hours , the nasal mucosa of the nasal respiratory part was measured . The nasal mucosa , the conventional tablets , the scanning electron microscope and the transmission electron microscope were used to observe the nasal mucosa of the nasal respiratory part .

【学位授予单位】：汕头大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R363

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