hsa-miR-197靶基因预测及生物信息学特征分析

发布时间：2018-01-12 01:35

本文关键词：hsa-miR-197靶基因预测及生物信息学特征分析　出处：《江苏医药》2014年04期 　论文类型：期刊论文

【摘要】：目的通过对hsa-miR-197进行系统的生物信息学分析,预测其靶基因、相关功能及其可能参与的生物学过程。方法通过PubMed检索hsa-miR-197相关文献;通过miRBase、NCBI等在线数据库获取并分析hsa-miR-197序列保守性及其基因组特征。通过GO功能注释、GO富集分析和信号转导通路富集分析,选择miRanda、TargetScan及PicTar预测hsa-miR-197的靶基因的交集作为分析的基因集合。结果 (1)hsa-miR-197在人类同源的物种间具有高度保守性。(2)三种方法预测结果取交集获得hsa-miR-197可能的靶基因共有32个。(3)hsa-miR-197靶基因主要参与DNA修复、蛋白磷酸化、离子转运、蛋白修饰、DNA依赖的转录调控等生物学过程,涉及Wnt、氨基糖类代谢等信号转导通路。结论 hsa-miR-197的功能较广泛,与发育、代谢等密切相关。
[Abstract]:Objective to predict the target gene of hsa-miR-197 by systematic bioinformatics analysis. Methods hsa-miR-197 literature was searched by PubMed. The conserved hsa-miR-197 sequence and its genomic characteristics were obtained and analyzed by on-line databases such as miRBaseNCBI. Go functional annotations were used. Go enrichment analysis and signal transduction pathway enrichment analysis were used to select miRanda. TargetScan and PicTar predict the intersection of target genes of hsa-miR-197 as an analytical gene set. Hsa-miR-197 is highly conserved among human homologous species.) three methods were used to predict the results. The possible target genes of hsa-miR-197 were obtained by intersection. Hsa-miR-197 target genes are mainly involved in DNA repair. Protein phosphorylation, ion transport, protein-modified DNA-dependent transcriptional regulation and other biological processes are involved in Wnt. Conclusion hsa-miR-197 has a wide range of functions and is closely related to development and metabolism.
【作者单位】：南京医科大学第二附属医院东院妇产科;南京医科大学附属南京市妇幼保健院妇科;
【基金】：国家自然科学基金青年科学基金(81202042)
【分类号】：R341
【正文快照】： 微小RNA(miRNA)是一类在真核生物体内发现的长度约22nt的内源性非编码小分子RNA,通过与靶标miRNA 3′端非编码区完全或不完全互补配对,从而降解靶标miRNA、抑制靶标miRNA翻译起始,在转录后水平进行基因调控,并参与调节细胞分化、增殖、凋亡、致癌、抑癌、激素分泌等大部分生物

【共引文献】