实验性神经症大鼠模型的建立与评价及电针干预的影响

发布时间：2018-01-12 14:27

本文关键词：实验性神经症大鼠模型的建立与评价及电针干预的影响　出处：《辽宁中医药大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的：建立实验性神经症大鼠模型，观察大鼠行为及中缝背核5-HT含量的变化规律，探讨电针对实验性神经症大鼠行为及中缝背核5-HT的影响。方法：健康成年SD大鼠50只，随机分为正常对照组、避暗实验组、基底节损毁组、电针治疗一组、电针治疗二组。5组动物均于两周后进行行为测试，测试后处死，常规灌注冰冻切片，采用SABC免疫组化和计算机图像分析技术，检测中缝背核5-HT神经元阳性细胞灰度值。结果：造模后，两组模型大鼠水平运动次数和垂直运动次数均明显降低，中缝背核内5-HT水平较正常组减低(P＜0.05)，电针干预后，治疗组5-HT含量较模型组有所增加。结论：1．避暗实验和基底节损毁法可成功建立神经症大鼠模型。2．模型组大鼠脑干中缝背核5-HT含量降低，与中医肝郁证有着共同的病理基础，均与体内单胺类神经递质及其生理活性相关。3．电针百会、印堂穴可使模型大鼠活动增多，中枢5-HT趋于正常，证明了电针百会、印堂可以改善模型动物的病理状态，从治疗手段有效性的反证角度，对两种模型的建立与评价提供了有力的支持。前言随着现代社会工作和生活节奏不断加快，竞争等应激事件日益增加，人们承受的心理压力也日益增大。无论是发达国家还是发展中的中国，心理健康问题越来越严重，其中又以神经症的发病率上升最为明显。神经症(neuroses)，旧称神经官能症，是临床医学中的重要疾病之一。本病为一组精神障碍，主要表现为：精神活动能力低下、烦恼紧张、焦虑抑郁、恐怖、强迫症状、疑病症状、分离症状、转换症状或各种躯体不适感。系统地研究神经症的生物学基础，有助于对神经症开展有针对性的预防和治疗。然而，由于对人类活体研究存在技术上和伦理上的限制，目前对本病的生物学机制仍知之甚少。因此，建立一个合理的动物模型实属必要。神经症当属祖国医学中“郁症”范畴。针灸治疗本类疾病在我国已有悠久的历史，早在《内经》时代就有针灸治疗本类疾病的记载，在历代文献记载中，约有130个穴位与治疗精神疾病有关，，如四关穴、十三鬼穴等。当今关于针灸治疗抑郁症、强迫症、焦虑症等神经症的报道更是不胜枚举。诸多研究显示：针灸治疗神经症的效果与临床一线药物相当，且具有无毒副作用、经济便捷的优势。本论文既以此为出发点，通过避暗实验和基底节损毁实验建立神经症大鼠模型，并采用反证法探讨电针治疗本病的作用机理，对模型的建立与评价提供有力支持。
[Abstract]:Objective: to establish a rat model of experimental neurosis and observe the changes of behavior and 5-HT content in dorsal raphe nucleus. To investigate the effect of electroacupuncture on the behavior of experimental neurosis rats and 5-HT in dorsal raphe nucleus. Methods: 50 healthy adult SD rats were randomly divided into normal control group, dark avoidance group and basal ganglion lesion group. The animals of the first group of electroacupuncture treatment and the 2 groups of electroacupuncture treatment group were given behavioral tests two weeks later. After the test, the animals were killed, frozen sections were infused routinely, and SABC immunohistochemistry and computer image analysis technique were used. Results: after the establishment of the model, the number of horizontal and vertical movements of the two groups were significantly decreased. The level of 5-HT in dorsal raphe nucleus was lower than that in normal group (P < 0.05). The 5-HT content in the treatment group was higher than that in the model group. Conclusion the neurotic rat model of neurosis can be established successfully by avoiding dark experiment and basal ganglia lesion. The content of 5-HT in the dorsal raphe nucleus of the brain stem of the model group is decreased. There is a common pathological basis with traditional Chinese medicine liver depression syndrome, which is related to monoamine neurotransmitters and their physiological activities. 3. EA Baihui, Yantang point can increase the activity of model rats, and the central 5-HT tends to normal. It is proved that electroacupuncture and Yantang can improve the pathological state of the model animals. From the point of view of the effectiveness of the therapeutic methods, it provides a strong support for the establishment and evaluation of the two models. Foreword With the rapid pace of work and life in modern society and the increasing number of stress events, such as competition, people are under increasing psychological pressure, whether in developed countries or in developing China. Mental health problems are becoming more and more serious, in which the incidence of neurosis is the most obvious. Neurosis, formerly known as neurosis. It is one of the most important diseases in clinical medicine. This disease is a group of mental disorders, mainly manifested in: low mental activity, anxiety and depression, anxiety and depression, phobia, compulsive symptoms, hypochondriac symptoms, separation symptoms. A systematic study of the biological basis of neurosis is helpful for targeted prevention and treatment of neurosis. Due to the technical and ethical limitations of human living research, little is known about the biological mechanism of the disease, so it is necessary to establish a reasonable animal model. Neurosis belongs to the category of "depression" in Chinese medicine. Acupuncture and moxibustion treatment of this kind of disease has a long history in our country, as early as in the "Internal Classic" era, acupuncture and moxibustion treatment of this kind of disease has been recorded in the literature records of the past dynasties. There are about 130 acupoints related to the treatment of mental illness, such as Siguan point, 13 ghost points, etc. Today about acupuncture treatment of depression, obsessive-compulsive disorder. There are numerous reports of anxiety disorders and other neuroses. Many studies show that acupuncture and moxibustion treatment of neurosis is as effective as clinical first-line drugs and has the advantages of no toxic side effects and convenient economy. This paper not only takes this as the starting point, through the dark avoidance experiment and the basal ganglia damage experiment to establish the neurosis rat model, and uses the counter-syndrome method to explore the mechanism of electroacupuncture treatment of this disease. Support the establishment and evaluation of the model.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R245

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