雄激素对去势大鼠下丘脑AR和NGF表达的影响

发布时间：2018-01-12 15:03

本文关键词：雄激素对去势大鼠下丘脑AR和NGF表达的影响　出处：《西北农林科技大学》2006年硕士论文　论文类型：学位论文

【摘要】： 为了探讨雄激素与脑组织之间的作用关系,本实验运用超敏感的免疫组织化学SP法,以SD大鼠为研究对象并建立去睾丸大鼠模型,通过补充外源性雄激素,观察脊髓颈膨大、下丘脑、嗅脑中AR和NGF的表达变化,探讨雄激素对脑中这两种物质的影响及其表达变化,研究雄激素对这些组织区域的保护机制。 1.在脊髓颈膨大中,AR和NGF免疫阳性产物广泛分布于脊髓的灰质中,而在白质中未见其阳性表达。在灰质中尤其以处于灰质背侧角的阳性细胞最为明显。AR阳性产物主要定位于细胞胞浆、胞膜和突起中,但核着色不明显。表明AR和NGF在脊髓中发挥了作用。但切除双侧睾丸后,AR表达强度没有明显下降,而NGF阳性表达强度则明显下降,注射外源性雄激素后,NGF阳性表达强度则恢复到假手术组水平。 2.AR和NGF在下丘脑中广泛分布,在下丘脑中的苍白球、弓状核、视前大细胞核、视前内侧核、视前外侧核、视上核、室旁核、室周核、下丘脑腹内侧核、下丘脑前核、下丘脑外侧核、终纹核等核团中均可见到AR和NGF的阳性表达。其阳性表达也主要定位于胞膜、胞质、突起中,在细胞核中仅有少量阳性表达。大鼠去睾丸后,体内内源性雄激素来源切断,下丘脑中大多核团AR和NGF阳性表达强度下降,在补充外源性雄激素后,上述变化被逆转,各核团中阳性细胞数目和阳性表达率均有所恢复。但苍白球中在切断内源性雄激素后,AR阳性表达强度有所上升,补充外源性雄激素后,阳性的表达强度有所下降。而观察的核团中,发现视上核在切断内源性雄激素后阳性细胞表达强度未见有大的变化,而在补充外源性雄激素后,依旧未见其阳性表达强度变化。 3.AR和NGF在嗅脑中也有广泛的分布,在嗅脑的僧帽细胞、嗅前核内侧部、内颗粒层、嗅结节丛状层等部位均可以观察到AR和NGF阳性细胞的表达。主要定位于胞浆和胞膜,在突起中也有少量分布。SD大鼠在去势后,由于缺乏内源性雄激素,嗅结节丛状层、嗅结节背侧部等区域AR和NGF阳性表达强度明显下降,在经补充雄激素治疗后,两种蛋白表达强度明显回升与假手术组相当。在嗅小球等部位,切断内源性雄激素后未见阳性细胞的表达,在补充外源性雄激素后,亦未见阳性细胞表达。
[Abstract]:In order to study the relationship between androgen and brain tissue, this experiment used the super-sensitive immunohistochemical SP method to establish the testicular rat model with SD rats as the object of study, through the supplement of exogenous androgen. To observe the changes of AR and NGF expression in cervical enlargement, hypothalamus and olfactory brain, to investigate the effects of androgen on these two substances and their expression, and to study the protective mechanism of androgen on these tissue regions. 1. AR and NGF immunoreactive products were widely distributed in the gray matter of spinal cord in cervical enlargement. In the gray matter, especially in the dorsal horn of gray matter, the positive products of AR were mainly located in the cytoplasm, membrane and process. But the nuclear staining was not obvious, which indicated that AR and NGF played an important role in spinal cord. However, after bilateral testicular resection, the expression of AR did not decrease significantly, but the positive expression of NGF decreased significantly. After injection of exogenous androgen, the positive expression of NGF returned to the level of sham operation group. 2. AR and NGF were widely distributed in hypothalamus, including globus pallidus, arcuate nucleus, preoptic nucleus, medial preoptic nucleus, anterolateral optic nucleus, supraoptic nucleus, paraventricular nucleus and periventricular nucleus. The positive expression of AR and NGF in ventromedial hypothalamic nucleus, anterior hypothalamic nucleus, lateral hypothalamic nucleus and stria terminalis nucleus can be seen. The positive expression of AR and NGF is mainly located in the membrane, cytoplasm and process. After removing testis, the endogenous androgen sources were cut off, and the intensity of AR and NGF expression in the hypothalamus decreased, after the supplementation of exogenous androgen. These changes were reversed, the number of positive cells and the positive expression rate in each nucleus recovered to some extent, but the intensity of AR positive expression in globus pallidus increased after transection of endogenous androgen, and after supplement of exogenous androgen. The positive expression intensity decreased. In the observed nuclei, there was no significant change in the expression intensity of the supraoptic nucleus after transection of endogenous androgen, but after the supplementation of exogenous androgen, there was no significant change in the expression intensity of the positive cells. There was no change in positive expression intensity. 3. AR and NGF were also widely distributed in olfactory brain, including mitral cells, medial part of olfactory anterior nucleus and inner granular layer of olfactory brain. The expression of AR and NGF positive cells were observed in the plexiform layer of olfactory nodules, mainly located in the cytoplasm and membrane, and a few distributed in the process. SD rats were ovariectomized. Due to the lack of endogenous androgen, the positive expression of AR and NGF in the plexiform layer of olfactory nodules and the dorsal part of the olfactory nodules decreased significantly, and the positive intensity of AR and NGF decreased significantly after androgen supplementation. The expression intensity of the two proteins was similar to that of the sham-operated group. No positive cells were found in the olfactory pellets after transection of endogenous androgen, and no positive cells were found after the supplementation of exogenous androgen.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

【引证文献】