插入序列ISAba1对多重耐药鲍曼不动杆菌碳青霉烯酶基因表达的影响

发布时间：2018-01-12 16:00

本文关键词：插入序列ISAba1对多重耐药鲍曼不动杆菌碳青霉烯酶基因表达的影响　出处：《中国抗生素杂志》2014年03期 　论文类型：期刊论文

【摘要】：目的了解耐碳青霉烯类鲍曼不动杆菌中插入序列ISAba1的存在对碳青霉烯酶基因表达的影响。方法筛选32株碳青霉烯类耐药的鲍曼不动杆菌,运用PCR技术检测OXA-23、OXA-24、OXA-51和OXA-58型酶基因及插入序列ISAba1;RT-PCR方法检测ISAba1下游基因的mRNA的相对表达水平。结果 OXA-51型基因的检出率为100%,OXA-23型基因为96.9%。其中23株OXA-23型基因、18株OXA-51型基因上游均存在插入序列。RT-PCR结果显示上游存在ISAba1时,OXA-23和OXA-51型基因相对表达水平分别为1.6471±0.6560和1.3209±0.3678,与不存在插入序列的相应基因表达水平相比,二者差异均有统计学意义(P0.001)。结论 OXA-23和OXA-51型基因是本组试验菌的主要碳青霉烯酶基因型,ISAba1序列的插入可引起下游碳青霉烯酶基因高表达。
[Abstract]:Objective to investigate the effect of inserted sequence ISAba1 on the expression of carbapenem gene in Acinetobacter baumannii. Methods 32 strains of Acinetobacter baumannii resistant to carbapenem were screened. OXA-23 OXA-24 OXA-51 and OXA-58 type enzyme gene and its insertion sequence ISAba1 were detected by PCR technique. RT-PCR method was used to detect the relative expression level of mRNA in ISAba1 downstream gene. Results the detection rate of OXA-51 gene was 100%. The type of OXA-23 gene was 96.9. of which 23 strains had OXA-23 type gene. The results of RT-PCR showed that there was ISAba1 in the upstream of all the 18 strains of OXA-51 gene. The relative expression levels of OXA-23 and OXA-51 were 1.6471 卤0.6560 and 1.3209 卤0.3678, respectively. Compared with the corresponding gene expression level without insertion sequence. Conclusion OXA-23 and OXA-51 genes are the main genotypes of carbapenem. The insertion of ISAba1 sequence can induce high expression of carbapenase gene downstream.
【作者单位】：川北医学院药物研究所暨药理学教研室;成都医学院结构特异性小分子药物研究实验室;
【基金】：国家自然科学基金资助项目(No.81373454) 四川省重点科技资助项目(No.2013jy0065)
【分类号】：R378
【正文快照】： 鲍曼不动杆菌是医院感染的重要条件致病菌,可引起各种严重感染。目前,碳青霉烯类抗菌药仍然是治疗其感染的最有效的药物之一,但有研究显示此类药物的广泛使用与鲍曼不动杆菌耐药性的增加具有相关性[1]。细菌一旦耐药,往往出现泛耐药甚至全耐药[2]。鲍曼不动杆菌对碳青霉烯

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