电化学疗法及微量生物样品介电常数测量的研究

发布时间：2018-01-12 18:07

本文关键词：电化学疗法及微量生物样品介电常数测量的研究　出处：《四川大学》2005年硕士论文　论文类型：学位论文

【摘要】：细胞膜是细胞外大分子进入细胞的主要屏障。电穿孔是指在瞬态电场作用下,瞬时增强细胞膜的通透性,在细胞膜的双脂层上形成瞬时可逆微孔的生物物理现象。由此发展起来的电穿孔技术在DNA转染、蛋白质导入、细胞电融合等方面都有良好应用。本文将其用于对离体细胞及恶性肿瘤的治疗中,对肿瘤注射化疗药物并施加电脉冲,通过肿瘤生长情况的检测,发现电脉冲结合化疗药物的电化学疗法可以极大地改善肿瘤治疗的效果。生物组织的介电常数是一个重要的物理量,它反映了组织在外场作用下的极化情况。在实际的病理诊断和临床治疗中,可以根据组织和器官的介质特性的变化和差异对病变组织进行诊断和分析,在肿瘤及其它病症的临床诊断和治疗中具有一定的应用前景。通常生物组织的取样数量十分有限,因此微量介质的介电常数测量在生物介质特性测量领域显得十分必要。谐振腔微扰法特别适合对微小体积样品的测试,很好解决了生物组织样品取样数量有限的问题。本文较详细地分析了谐振腔微扰法,并由谐振腔微扰理论出发,得到介质复介电常数计算式。针对生物组织高含水量、高介电常数、高损耗的特性,采取了加大谐振腔体积、减小样品体积及提高对样品体积测试精度等措施,以提高生物样品介电常数的测试精度。通过对水和不同浓度NaCl溶液介电常数的测试,验证了本系统的测量精度较高。本文首次对电脉冲处理下的肿瘤细胞悬液、小鼠血液的复介电常数进行了测量,对电磁场生物效应的研究具有一定参考价值,对组织介质特性在临床上的检测有一定前瞻性的意义。
[Abstract]:The cell membrane is the main barrier of extracellular macromolecules into cells. Electroporation refers to the transient electric field, instantaneous increase of cell membrane permeability, biophysical phenomenon of transient reversible micropores in the bilayer of the cell membrane. The electroporation technique developed in the DNA transfected into protein, cell electrofusion etc. have a good application. This paper will be used in the treatment of malignant tumor cells in vitro and in tumor chemotherapy and applying electric pulses, detection of tumor growth through, found that electric pulse combined with electrical chemical therapy chemotherapy can greatly improve the effects of cancer treatment.
The permittivity of biological tissues is an important physical quantity, it reflects the polarization organization under the action of external field. The pathological diagnosis and clinical treatment of the actual, can the diagnosis and analysis of lesions according to the change of dielectric properties of tissues and organs and differences, has a certain application prospect in clinical diagnosis and for the treatment of tumors and other diseases. Usually very limited sampling quantity of biological tissues, so the trace of dielectric constant measurement in biological field measurement of dielectric properties is very necessary. Particularly suitable for testing small volume sample cavity perturbation method, solves the problem of biological tissue samples of a limited number of sampling.
This thesis makes a detailed analysis of the resonant cavity perturbation method, and based on perturbation theory, obtained the complex dielectric constant formula. According to the biological tissue high moisture content, high dielectric constant, high loss characteristics, to increase the cavity volume, reduce the sample volume and improve the testing precision of sample volume such measures, in order to improve the testing precision of biological samples. Based on the dielectric constant of water and different concentrations of NaCl solution dielectric constant test, verify the measurement precision of the system. This is the first research on the electric pulse treatment of tumor cell suspension, the complex permittivity of mice blood were measured with the reference value to the study of biological effect of electromagnetic field, has certain forward-looking significance to detection of tissue dielectric properties in clinic.

【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R35;R730.5

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