脓毒症大鼠血小板生成素水平与脓毒症严重程度的关系

发布时间：2018-01-13 08:01

本文关键词：脓毒症大鼠血小板生成素水平与脓毒症严重程度的关系　出处：《兰州大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的观察二次打击致脓毒症大鼠血清血小板生成素(TPO)水平的改变,并初步探讨脓毒症时血清TPO水平与脓毒症严重程度的关系。方法将SD大鼠分成健康对照组(A,n=10)、手术对照组(B,n=8)、脓毒症组(C,n=15)、严重脓毒症组(D,n=12)和脓毒性休克组(E,n=8)。通过低血容量休克打击后注射不同剂量(1mg/kg、2mg/kg、3mg/kg)大肠杆菌内毒素(LPS)复制脓毒症、严重脓毒症及脓毒性休克模型。24小时后测定大鼠血小板计数和血清TPO、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果脓毒症组血小板较健康对照组无明显变化,严重脓毒症组和脓毒性休克组血小板计数下降显著(P0.01)。TPO水平在脓毒症组开始升高(P0.05),在严重脓毒症组和脓毒性休克组显著升高(P0.01)。IL-6、TNF-α在脓毒症组、严重脓毒症组和脓毒性休克组逐渐升高。多元线性回归分析显示:TPO与IL-6密切相关(r~2=0.832,P0.00)。结论脓毒症大鼠血清TPO水平升高,脓毒症严重程度是TPO水平主要决定因素,TPO水平可作为脓毒症严重程度的生物学标志。
[Abstract]:Objective To observe the changes of serum thrombopoietin ( TPO ) levels in rats with sepsis and to investigate the relationship between serum TPO levels and severity of sepsis in sepsis . Methods SD rats were divided into healthy control group ( A , n = 10 ) , control group ( B , n = 8 ) , sepsis group ( C , n = 15 ) , severe sepsis group ( D , n = 12 ) and septic shock group ( E , n = 8 ) . The levels of platelet count and serum TPO , interleukin - 6 ( IL - 6 ) and tumor necrosis factor - 伪 ( TNF - 伪 ) in rat platelets were determined by low - blood volume shock after injection of different doses ( 1 mg / kg , 2 mg / kg , 3 mg / kg ) of E . coli endotoxin ( LPS ) . Results There was no significant change in platelet count in septic group and septic shock group ( P0.01 ) . The levels of TPO increased significantly in septic group ( P0.05 ) . The levels of IL - 6 and TNF - 伪 increased gradually in septic group , severe sepsis group and septic shock group . Conclusion The serum TPO level in septic rats is elevated , the severity of sepsis is the main determinant of TPO level , and TPO level can be used as a biological marker for sepsis severity .

【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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