乙肝病毒感染性疾病与Fas系统相关性研究

发布时间：2018-01-14 02:02

本文关键词：乙肝病毒感染性疾病与Fas系统相关性研究　出处：《青岛大学》2006年硕士论文　论文类型：学位论文

更多相关文章： 乙肝病毒 细胞凋亡 Fas FasL

【摘要】： 目的通过对乙型肝炎病毒感染相关性疾病患者外周血中淋巴细胞凋亡情况及细胞膜表面Fas／FasL的检测，结合乙肝病毒感染后患者血清中可溶性Fas／FasL(sFas／sFasL)水平的改变，探讨在疾病发生发展过程中，淋巴细胞表面所表达的Fas／FasL与血清sFas／sFasL之间的变化规律，期望进一步阐明乙肝病毒感染慢性肝细胞损害致重症化和肝细胞癌变的发病机制，为预防和治疗慢性乙型重型肝炎和肝细胞癌提供新的思路。方法采用流式细胞技术检测健康人、乙肝病毒携带者、慢性乙型重型肝炎患者、乙肝病毒相关性肝细胞癌患者外周血淋巴细胞的凋亡率及细胞表面表达Fas／FasL的情况；采用双抗体夹心法检测患者和健康人血清中sFas／sFasL的表达。结果(1)通过流式细胞技术检测证实，与正常对照组、无症状乙肝病毒携带组相比较，慢性乙型重型肝炎患者、乙肝病毒相关性肝细胞癌患者外周血淋巴细胞的凋亡率显著增高，差别有显著性(P＜0.01)。(2)通过流式细胞技术检测证实，与正常对照组、无症状乙肝病毒携带组相比较，慢性乙型重型肝炎患者、乙肝病毒相关性肝细胞癌患者外周血淋巴细胞表面表达的Fas显著增高，差别有统计学意义(P＜0.01)；与正常对照组、无症状乙肝病毒携带组相比较，慢性乙型重型肝炎患者外周血淋巴细胞表面表达的FasL显著增高，差别有统计学意义(P＜0.01)，但是，，乙肝病毒相关性肝细胞癌患者外周血淋巴细胞表面表达的FasL显著降低，与其他三组相比较，差别有统计学意义(P＜0.01)。(3)双抗体夹心法证实，与正常对照组、无症状乙肝病毒携带组相比较，慢性乙型重型肝炎患者血清sFas和sFasL含量均显著增高，差别有显著性(P＜0.01)；乙肝病毒相关性肝细胞癌患者sFas含量明显增高，sFasL含量显著降低，差别有统计学意义(P＜0.01)。结论(1)慢性乙型重型肝炎患者、乙肝病毒相关性肝细胞癌患者外周血淋巴细胞的凋亡率显著增高。(2)Fas／FasL在慢性乙型重型肝炎患者和乙肝病毒相关性肝细胞癌患者外周血淋巴细胞中的表达存在明显差异。(3)sFas和sFasL在慢性乙型重型肝炎及乙肝相关性肝细胞癌之间存在明显差异。(4)Fas／FasL和sFas／sFasL的相互作用在淋巴细胞的凋亡中发挥着重要的作用，与慢性乙型重型肝炎、乙肝病毒相关性肝细胞癌的发病密切相关。
[Abstract]:Objective to detect the infection of hepatitis B virus in patients with diseases associated with apoptosis of lymphocytes in cell membrane and the surface of Fas / FasL, in combination with hepatitis B virus infection after soluble in the serum of patients with Fas / FasL (sFas / sFasL) level change, to explore in the disease process, variation of lymphocyte surface expression Fas / FasL and sFas / sFasL in serum, pathogenesis and further clarify the expectations caused by severe hepatic cell canceration of chronic liver damage in hepatitis B virus infection, to provide new ideas for the prevention and treatment of chronic severe hepatitis and hepatocellular carcinoma.
Methods using flow cytometry to detect hepatitis B virus carriers, healthy people, patients with chronic severe hepatitis B, peripheral blood lymphocyte surface of hepatitis B virus in patients with hepatocellular carcinoma: correlation between the apoptosis and the expression of Fas / FasL; the expression was determined by ELISA in patients and healthy human serum in sFas / sFasL.
Results (1) confirmed by the detection of flow cytometry, and the normal control group, asymptomatic HBV carriers group compared to patients with chronic severe hepatitis B, apoptosis of peripheral blood lymphocytes in patients with hepatitis B virus related hepatocellular carcinoma was significantly increased, the difference was significant (P < 0.01). (2) by detecting confirmed by flow cytometry, and the normal control group, asymptomatic HBV carriers group, chronic severe hepatitis B patients, surface expression of peripheral blood lymphocytes in patients with hepatitis B virus related hepatocellular carcinoma Fas was significantly increased, the difference was statistically significant (P < 0.01); and the normal control group, asymptomatic hepatitis B virus carrying group compared to FasL lymphocyte surface expression in peripheral blood of patients with chronic severe hepatitis B were significantly higher, the difference was statistically significant (P < 0.01). However, patients with hepatitis B virus related hepatocellular carcinoma peripheral blood lymphocytes Cell surface expression of FasL was significantly decreased compared with the other three groups, the difference was statistically significant (P < 0.01). (3) confirmed by double antibody sandwich method, and the normal control group, asymptomatic HBV carriers group, chronic severe hepatitis B patients serum sFas and sFasL levels were significantly increased, the difference is significant (P < 0.01); the content of sFas of hepatitis B virus related hepatocellular carcinoma patients were significantly increased, sFasL content decreased significantly, the difference was statistically significant (P < 0.01).
Conclusion (1) in patients with chronic severe hepatitis B, apoptosis of peripheral blood lymphocytes in patients with hepatitis B virus related hepatocellular carcinoma was significantly increased. (2) Fas / FasL in patients with chronic severe hepatitis B and hepatitis B virus related hepatocellular carcinoma in peripheral blood lymphocytes was significantly different. (3) and sFas there were significant differences in sFasL between chronic hepatitis B and hepatitis B virus associated hepatocellular carcinoma. (4) the interaction between Fas / FasL and sFas / sFasL plays an important role in lymphocyte apoptosis, and chronic severe hepatitis B, closely related to the pathogenesis of hepatitis B virus associated hepatocellular carcinoma.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

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