急性热应激对大鼠中脑和脑桥Fos和GFAP表达的影响

发布时间：2018-01-14 17:25

本文关键词：急性热应激对大鼠中脑和脑桥Fos和GFAP表达的影响　出处：《第四军医大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的探讨急性热应激后大鼠中脑导水管周围灰质与脑桥臂旁核Fos与GFAP的表达情况。方法将大鼠分别置于24℃、34℃、38.5℃或42℃、湿度为60%的恒温恒湿温度舱内1 h,建立急性热应激大鼠模型,应用免疫组织化学染色检测中脑导水管周围灰质与脑桥臂旁核Fos蛋白和胶质原纤维酸性蛋白(GFAP)表达情况。结果1.对照组大鼠中脑内的Fos阳性神经元很少。34℃组Fos阳性神经元有所增加。38.5℃组神经元的反应达高峰。42℃组神经元反应减弱。在实验组,Fos阳性神经元主要分布于中脑导水管周围灰质,尤其是腹外侧区。2.在中脑,GFAP阳性星形胶质细胞的分布区域与Fos阳性神经元类似。24℃组,GFAP阳性星形胶质细胞显示静息特点,胞体小,突触细长;而34℃组表现为活化型,胞体变大,突起增粗,细胞数量增加,GFAP染色加深;38.5℃组最显著,42℃组则出现胞体形态不规则,突起断裂。3.脑桥臂旁核内可见Fos阳性神经元,其中在24℃～38.5℃组Fos蛋白的表达随温度增高而增加,38.5℃组为高峰,而在42℃组Fos蛋白表达又有所减少。4.脑桥内GFAP阳性星形胶质细胞的分布特点与Fos阳性神经元类似,24℃组,GFAP阳性星形胶质细胞显示静息特点,胞体小,突触细长;在34℃组表现为活化型,胞体变大,突起增粗的现象,且细胞数量增加,GFAP染色加深;38.5℃组最显著,但在42℃组星形胶质细胞的数量则有所减少,并出现胞体形态不规则、突起断裂的现象。结论1.中脑导水管周围灰质,尤其是腹外侧区神经元、星形胶质细胞参与热应激反应的神经免疫调节,有可能为此调节通路的中继站之一。2.脑桥臂旁核参与热应激反应的神经免疫调节,也可能为此调节通路的中继站之一。3.在本实验中38.5℃是机体保持正常反应的极限温度,在此温度以下,神经元和星形胶质细胞的反应随温度上升而加强,温度升至42℃时神经元和神经胶质细胞受损伤,蛋白表达又出现减少。
[Abstract]:Objective to investigate the expression of acute heat stress in rat midbrain periaqueductal gray and parabrachial nuclei of Fos and GFAP. Methods the rats were placed in 24 degrees, 34 degrees, 38.5 degrees or 42 degrees Celsius temperature, humidity and constant temperature and humidity cabin in 60% of the 1 h, to establish a rat model of acute heat stress, immune histochemical staining of periaqueductal gray and parabrachial nucleus Fos protein and glial fibrillary acidic protein (GFAP) expression. Results of the 1. rats in the control group in midbrain Fos positive neurons rarely.34 Fos positive neurons increased in group C.38.5 C group neuronal responses peaked at.42 DEG neurons decreased. In the group the experimental group, Fos positive neurons were mainly distributed in the periaqueductal gray matter, especially the ventrolateral.2. in the midbrain, GFAP positive astrocytes and Fos positive neurons in the distribution area of similar.24 C group, GFAP positive astrocytes Glial cells showed characteristics of resting, small cell body, and synaptic slender; 34 C group showed activation, larger cell body, neurite thickening, increase in the number of cells, GFAP staining deepened; the most significant 38.5 C group, 42 C group showed cell morphology was irregular, bulge fracture.3. parabrachial nuclei in the Fos positive neurons, the expression in the 24 to 38.5 DEG C group of Fos protein increased with the temperature of 38.5 DEG C, group peak, and it reduced the expression of Fos and distribution of GFAP positive astrocytes and.4. positive neurons in the pons were similar between the 42 group of Fos protein C, 24 C group, GFAP positive astrocytes glial cells showed characteristics of resting, small cell body, synaptic slender; at 34 DEG C group showed activation, larger cell body, protruding thickening phenomenon, and the increase in the number of cells, GFAP staining deepened; the most significant 38.5 C group, but in 42 the number of C group of astrocytes decreased, and the emergence of The cell body of irregular shape, protruding fracture phenomenon. Conclusion 1. periaqueductal gray matter, especially the ventrolateral neurons, neural immune astrocytes participate in the regulation of heat stress response, may relay station of this regulated pathway of.2. parabrachial nuclei involved in the heat shock response should neuro immune regulation may therefore the relay station of.3. pathway in this experiment is 38.5 DEG C temperature limit of the body to keep normal reaction, below this temperature, the response of neurons and astrocytes with temperature rise and the temperature rise to 42 degrees to strengthen, neurons and glial cells were damaged, and the protein expression decreased.

【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

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