肺炎克雷伯杆菌对氟喹诺酮类抗生素耐药机制的研究

发布时间：2018-01-14 21:29

本文关键词：肺炎克雷伯杆菌对氟喹诺酮类抗生素耐药机制的研究　出处：《四川大学》2006年硕士论文　论文类型：学位论文

【摘要】：肺炎克雷伯杆菌是一种常见的条件致病菌，近年来，引起医院内感染的发病率越来越高，尤其是儿童、老年人和抵抗力低的人群。喹诺酮类抗菌素作为治疗肺炎克雷伯杆菌感染的常用药，由于广泛地使用，甚至滥用，耐药性也呈逐年升高的趋势，给临床治疗带来了很大的困难，甚至治疗失败。研究耐喹诺酮肺炎克雷伯杆菌对其它抗菌素的抗菌活性及对氟喹诺酮耐药机制的分析，对临床治疗耐喹诺酮肺炎克雷伯杆菌引起的感染和开发新的喹诺酮类药物有着重要意义。为了研究耐喹诺酮肺炎克雷伯杆菌对其他抗菌素的抗菌活性，从100株肺炎克雷伯杆菌中选取对环丙沙星耐药(MIC≥4mg／L)菌株14株。采用琼脂二倍稀释法测定对抗菌素的最低抑菌浓度(MIC)，同时做了泵抑制剂羰基氰氯苯腙(CCCP)的逆转试验。MIC测定结果显示，，14株肺炎克雷伯杆菌对5种喹诺酮类药物无一株敏感，且表现出较高水平的耐药。对其它抗生素的耐药率由高到低依次是氨苄西林、哌拉西林、氯霉素、庆大霉素、氨曲南、阿米卡星、头孢噻肟和头孢吡肟，而对于亚胺培南未发现有耐药株。
[Abstract]:Klebsiella pneumoniae is a common opportunistic pathogen, in recent years the incidence of nosocomial infection is more and more high, especially children, the elderly and people with low resistance. Quinolones as a treatment for Klebsiella pneumoniae Klebsiella pneumoniae infection commonly used drugs, because of widely use, even abuse. Drug resistance also showed an increasing trend, has brought great difficulties to the clinical treatment, and treatment failure. The antibacterial activity of quinolone resistant Klebsiella pneumoniae Klebsiella pneumoniae to other antibiotics and analysis of the mechanism of fluoroquinolone resistance in the clinical treatment of quinolone resistant Klebsiella pneumoniae infection and the development of new quinolones have of great significance.
In order to study the antibacterial activity of quinolone resistant Klebsiella pneumoniae to other antibiotics, from 100 strains of Klebsiella pneumoniae were resistant to ciprofloxacin (MIC = 4mg / L) 14 strains. The determination of minimum inhibitory concentration of antibiotics by two fold agar dilution method (MIC), the pump inhibitor carbonyl cyanide m-chlorophenylhydrazone at the same time hydrazone (CCCP) determination results of reversal test of.MIC showed that 14 strains of Klebsiella pneumoniae to 5 quinolones without a strain sensitive, and showed high level resistance to other antibiotics. The resistance rate from high to low is ampicillin, piperacillin, chloramphenicol, gentamicin, aztreonam Amikacin, cefotaxime and cefepime and imipenem for no resistant strain was found.

【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R378

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