龙岩市手足口病病原体柯萨奇B5病毒基因特征分析

发布时间：2018-01-15 02:20

本文关键词：龙岩市手足口病病原体柯萨奇B5病毒基因特征分析　出处：《中国病原生物学杂志》2014年11期 　论文类型：期刊论文

【摘要】：目的了解龙岩市手足口病病原体柯萨奇B5病毒(Coxsackie virus B5,CVB5)VP1区基因特征及变异情况。方法对2011年采自确诊手足口病患者的Real-time RT-PCR法检测肠道病毒通用引物阳性而肠道病毒71型(enterovirus 71,EV 71)和柯萨奇病毒A 16型(Coxsackie virus A 16,CVA16)阴性未分型样品,经RD细胞分离肠道病毒。从培养上清中提取RNA核酸,经RT-PCR法初步鉴定病毒型别。应用187/222引物对扩增病毒株的VP1区,回收产物经克隆、筛选后测序。采用ClustalX、Mega5.0进行核苷酸及氨基酸序列同源性分析和进化树构建。结果 104份未分型样品中分离到3株CVB5。3株CVB5分离株VP1区长度为330~357个核苷酸,编码110~119个氨基酸。3株间的核苷酸同源性为97%~99%,氨基酸同源性为84%~99%。比较推导的氨基酸序列,FJLY2011126h株VP1与另外2株龙岩分离株相比有18个位点差异,核苷酸序列287~288位发生插入突变,第290、291、338、339位发生颠换,与2010年长春株JN695051的同源性最高(核苷酸同源性95%~98%,氨基酸同源性84%~99%)。结论 2011年福建省龙岩市手足口病患者中存在CVB5感染,病毒与2005年法国株和国内山东、长春、昆明、浙江病毒株属同一型别。
[Abstract]:Objective to understand the Longyan City HFMD pathogen of Coxsackie virus B5 (Coxsackie virus B5, CVB5) gene characteristics and variation of VP1. Methods collected from HFMD patients diagnosed with Real-time RT-PCR method for detection of enterovirus universal primers was enterovirus 71 on 2011 (enterovirus 71, EV 71) and Coxsackie virus A type 16 (Coxsackie virus 16 A, CVA16) negative unclassified samples by RD cells isolated from intestinal viruses. From the culture supernatant of RNA nucleic acid extraction, identified by RT-PCR method. The application of 187/222 type virus primer pairs amplified virus strains VP1, recovery of product was cloned, sequenced. After screening by ClustalX, Mega5.0 nucleotide and amino acid sequence homology analysis and phylogenetic tree construction. Results 104 undefferentiated were isolated 3 strains of CVB5.3 CVB5 isolates VP1 length of 330~357 nucleotides, encoding 110~119 amino acids of.3 strains The nucleotide homology was 97%~99%, amino acid homology comparison of the deduced amino acid sequence of 84%~99%. FJLY2011126h strain VP1, and the other 2 Longyan isolates compared with 18 loci between a nucleotide sequence of 287~288 insertion mutation, 290291338339th occurred in 2010 and Changchun transversion, strain JN695051 had the highest homology (nucleotide homology of 95%~98%. The homology of amino acid 84%~99%). Conclusion the 2011 Longyan city of Fujian province HFMD patients are infected by CVB5 virus, and in 2005 the French line and domestic Shandong, Kunming, Zhejiang Changchun, strains belonging to the same type.

【作者单位】：龙岩市疾病预防控制中心;福建医科大学龙岩市疾病预防控制中心教学点;
【基金】：福建省卫生厅青年科研课题项目(No.2012-1-47)
【分类号】：R373
【正文快照】： ***2011年龙岩市共报告手足口病(hand-foot-mouthdisease,HFMD)病例6 701例,其中重症病例13例,死亡病例1例。对采集的302份HFMD病例咽拭标本进行病毒核酸检测(Real-time RT-PCR法),肠道病毒通用引物核酸阳性207份(68.54%),其中EV 71核酸阳性46份(15.23%),CVA16核酸阳性57份(18

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