大鼠重度闭合性颅脑损伤模型构建及伤后S100β蛋白表达与脑损伤时间关系的研究

发布时间：2018-01-15 12:20

本文关键词：大鼠重度闭合性颅脑损伤模型构建及伤后S100β蛋白表达与脑损伤时间关系的研究　出处：《延边大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的：改进Marmarou法制作大鼠重度弥漫性脑损伤模型，观察大鼠重度弥漫性脑损伤后S100β蛋白在脑组织中不同部位的时间相关性表达，并以此为弥漫性脑损伤时间推断、生前伤和死后伤判断及早期诊断提供实验依据。方法：使用改进后的Marmarou致伤装置，，以冲击力(180cm×450g)打击大鼠头部制作重度弥漫性颅脑损伤动物模型，观察损伤后动物神经系统体征及脑组织病理形态学变化。应用免疫组织化学方法和图像分析技术，检测弥漫性脑损伤后30min，2h，4h，12h，24h，3d，7d，死后10min后损伤，各组S100β蛋白在大脑皮质、海马、丘脑和脑干中神经胶质细胞的表达，并设立正常组作对照。结果：(1)与对照组相比，损伤后大鼠神经系统体征有明显改变，病理学检查各致伤组动物均有弥漫性脑损伤。(2)与对照组相比，S100β阳性细胞个数及蛋白表达在皮质、海马和丘脑形成先增后降再升的曲线，即两次表达高峰，脑干表达变化不显著；在伤后2h，海马、丘脑S100β表达细胞个数和蛋白量开始增加，4h有显著增加，12h达到高峰值，皮质部位在4h达到高峰。伤后7天恢复正常值。结论：(1)改进后的大鼠弥漫性脑损伤模型稳定、实用、重复性好；打击伤组动物动物死亡率低且病理变化典型，适合动态研究弥漫性脑损伤后的病理生理变化。(2)弥漫性脑损伤后S100β在不同部位表达有不同规律性且有较好的时间相关性，可成为法医脑损伤时间推断的一种有效指标。
[Abstract]:Objective: to establish the rat model of diffuse brain injury with modified Marmarou method, were observed in rats with severe diffuse brain injury after S100 beta protein in the brain tissue of time correlation in different parts of the expression, and as a diffuse brain injury time estimation, antemortem and after injury and provide experimental basis for early stage diagnosis of judgment.
Methods: using the improved Marmarou injury device, the impact force (180cm * 450g) on the rat head produced severe diffuse brain injury animal model, the pathological changes of brain tissue and animal signs of nervous system injury were observed. Immunohistochemical method and image analysis technology, 30min detection of diffuse brain injury. After 2h, 4h, 12h, 24h, 3D, 7d, 10min after death after injury, the S100 protein in the cerebral cortex, hippocampus, thalamus and the expression of glial cells in the brain stem, and the establishment of normal group as control.
Results: (1) compared with the control group, after the injury of the rat nervous system signs changed obviously, pathological examination of each animal injury group had diffuse brain injury. (2) compared with the control group, the expression of S100 beta positive cell number and protein in cortex, hippocampus and thalamus form falling or curve first after the increase, which is two times the peak expression did not change significantly, the expression of brain stem; hippocampus at 2h after injury, the expression of S100 in thalamus, the cell number and protein content began to increase, 4H increased significantly, 12h reached the peak, the cortex peaked at 4H. 7 days after injury to restore normal values.
Conclusion: (1) improved after diffuse brain injury model in rats is stable, practical, good repeatability; combat injury group of animal mortality rate is low and the typical pathological changes, suitable for the dynamic study of the pathophysiological changes after diffuse brain injury. (2) S100 after diffuse brain injury in different parts have different expression of beta the regularity and the time correlation, can become an effective index of time forensic brain injury inference.

【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R651.15

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