Ⅰ.5-羟色胺、5-羟色胺2A受体参与调节呼吸中枢pre-B（？）tzinger complex的初步研究 Ⅱ.卵巢切除

发布时间：2018-01-15 16:40

本文关键词：Ⅰ.5-羟色胺、5-羟色胺2A受体参与调节呼吸中枢pre-B（？）tzinger complex的初步研究 Ⅱ.卵巢切除术后大鼠垂体前叶生长相关蛋白-43免疫反应神经纤维表达增加　出处：《第四军医大学》2006年硕士论文　论文类型：学位论文

【摘要】：本文由两部分构成。第一部分是关于成年大鼠5-羟色胺、5-羟色胺2A受体参与中枢呼吸调节的初步研究。第二部分是关于卵巢切除术后大鼠垂体前叶生长相关蛋白-43免疫反应性及其与促性腺激素细胞的关系。位于新生和成年哺乳动物延髓腹外侧区的前包钦格复合体(pre-B(?)tzinger complex，pre-B(?)tC)被认为是呼吸节律产生中枢。5-羟色胺(5-hydroxytryptamine，5-HT)作为一种重要的神经递质，在感觉和运动功能的调控中发挥关键作用，其中也包括对呼吸节律的调节。基于目前诸多不同的电生理实验结果而又缺乏基本的、必要的形态学实验基础，我们试图阐明5-HT、5-HT_(2A)受体(5-HT_(2A) receptor，，5-HT_(2A)R)与pre-B(?)tC神经元之间的关系，为5-HT参与呼吸节律的调控提供形态学实验依据。神经激肽1受体(neurokinin-1 receptor，NK1R)已经被广泛地用做pre-B(?)tC神经元的特异性标记物。本实验应用NK1R标记pre-B(?)tC神经元，采用免疫荧光组织化学和ABC法结合免疫金-银加强的免疫电镜双重标记技术，分别观察了成年正常和慢性间断性缺氧(chronic intermittent hypoxia，
[Abstract]:This paper consists of two parts. The first part is about the 5- of adult rat serotonin, a preliminary study on central respiratory regulation in 5- serotonin 2A receptor. The second part is about the anterior pituitary of rats following ovariectomy growth associated protein -43 immunoreactivity and its relationship with gonadotrophs.
In neonatal and adult mammalian ventrolateral medulla of the pre botinger complex (pre-B (?) tzinger complex, pre-B (?) tC) is considered to be the respiratory rhythm of central serotonin.5- (5-hydroxytryptamine, 5-HT) as an important neurotransmitter, play a key role in the regulation of sensory and motor function in the including the regulation of respiratory rhythm. Based on many different electrophysiological experiments and the lack of basic morphology, experimental basis necessary, we tried to clarify the 5-HT, 5-HT_ (2A) receptor (5-HT_ (2A) receptor, 5-HT_ (2A) R) and pre-B (?) tC relationship between neurons, morphology the experimental basis for 5-HT involved in regulation of respiratory rhythm. Neurokinin 1 receptor (neurokinin-1 receptor NK1R) has been widely used for pre-B (?) tC specific neuronal marker. The experimental application of NK1R marker pre-B (?) tC neurons by Immunofluorescence histochemistry and ABC combined with immunogold silver enhanced immunoelectron microscopy double labeling technique were used to observe adult normal and chronic intermittent hypoxia (chronic intermittent hypoxia, respectively).

【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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