结核分枝杆菌L型的诱导及其对抗菌药物的敏感性

发布时间：2018-01-16 21:36

本文关键词：结核分枝杆菌L型的诱导及其对抗菌药物的敏感性　出处：《山东医药》2014年39期 　论文类型：期刊论文

【摘要】：目的观察异烟肼、利福平、乙胺丁醇抗结核药物诱导形成结核分支杆菌L型以及结核分支杆菌L型对抗菌药物敏感性。方法将结核分支杆菌接种于含有不同质量浓度利福平(0.05、0.1、0.2μg/m L)、异烟肼(0.01、0.04、0.08μg/m L)、乙胺丁醇(1、2.5、5μg/m L)的BD960液体培养基内,10 d后在显微镜下观察结核分支杆菌L型的形成情况。培养结核分支杆菌L型,在显微镜高倍镜下观察,并检测其对抗菌药物的敏感性。结果 0.1、0.2μg/m L利福平,0.04、0.08μg/m L异烟肼,2.5、5μg/m L乙胺丁醇可诱导形成结核分支杆菌L型。在含有4μg/m L链霉素、0.2μg/m L异烟肼、40μg/m L利福平、2μg/m L乙胺丁醇、1μg/m L对氨基水杨酸的非高渗培养基内培养7 d后均可见结核分枝杆菌L型生长,而含有30μg/m L卡那霉素、40μg/m L卷曲霉素、3μg/m L氧氟沙星的培养基内未见其生长。结论中高浓度的利福平、异烟肼、乙胺丁醇能够诱导结核分支杆菌L型形成。结核分枝杆菌L型对卡那霉素、卷曲霉素、氧氟沙星敏感。
[Abstract]:Objective to observe isoniazid and rifampicin. Methods Mycobacterium tuberculosis was induced to form mycobacterium tuberculosis L form and Mycobacterium tuberculosis L form was sensitive to antimicrobial agents. Methods Mycobacterium tuberculosis was inoculated with rifampicin of different concentrations. 0.1U 0.2 渭 g / m L ~ (-1), 0.011 渭 g / m ~ (-1) of isoniazid, 0.08 渭 g / m ~ (-1) L ~ (-1) of ethambutanol, 2.5 渭 g / m ~ (-1) of ethambutanol. The formation of mycobacterium tuberculosis L-form was observed under microscope after 10 days in 5 渭 g / mL BD960 liquid medium. The L-form of mycobacterium tuberculosis was cultured and observed under high power microscope. The sensitivity to antimicrobial agents was determined. Results 0.1 渭 g / mL rifampicin 0.08 渭 g / mL isoniazid 2.5 was used. The L-form of Mycobacterium tuberculosis was induced by 5 渭 g / mL ethambutanol and 40 渭 g / mL rifampicin containing 4 渭 g / mL streptomycin 0.2 渭 g / mL isoniazid. 2 渭 g / mL ethambutanol 1 渭 g / mL in non-hypertonic culture medium of aminosalicylic acid for 7 days all showed L-form growth of Mycobacterium tuberculosis, but contained 30 渭 g / m L kanamycin. There was no growth of ofloxacin in 40 渭 g / mL ofloxacin 3 渭 g / mL ofloxacin. Conclusion Rifampicin and isoniazid at medium and high concentrations were not found. The L-form of Mycobacterium tuberculosis is sensitive to kanamycin, capramycin and ofloxacin.
【作者单位】：天津市海河医院;
【基金】：天津市卫生局科技基金项目(2013KY13)
【分类号】：R378.911
【正文快照】： 结核病严重影响人类健康,抗结核药物的出现使结核病得到有效控制,但近10年,结核病疫情迅速回升,尤其耐药结核病的流行,为结核病疫情的控制带来更大的挑战。有研究[1,2]表明,结核分枝杆菌菌壁缺失变异形成L型后可长期潜伏于宿主体内导致结核病复燃。同时有研究[3]表明,结核分枝

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