体外培养人树突状细胞脂质体介导转染HBcAg基因的实验研究

发布时间：2018-01-19 00:34

本文关键词： 肝炎病毒乙型肝炎核心杭原乙型树突状细胞脂质体转染　出处：《南京医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：乙型肝炎病毒(hepatitis B virus HBV)感染是一个世界范围内的高感染率、高危害性的疾病：全球约有3.5亿HBV感染者，仅我国乙肝病毒感染率就达约60-70％，其中15-25％感染者最终会死于HBV相关性疾病，这使得HBV感染成为危害人类最严重的疾病之一。目前，抗乙肝病毒的主要治疗药物为干扰素和核苷类似物拉米夫定等，，但是由于二者均不能消除病毒复制模板共价闭合环状DNA(cccDNA)的存在，故停药后病情仍会反复。因此对于慢性HBV感染治疗上的深入研究有着极强的现实意义。现有的研究发现在持续性HBV感染的发病机制中，特异性细胞免疫能力的低下或不足起着至关重要的作用，而树突装细胞(DC)作为目前已知的人体内最强大的抗原递呈细胞，机体免疫反应的始动者，通过参与抗原的摄取加工与递呈，对诱导免疫应答起着关键性的作用，与慢性乙型肝炎的发病机理和治疗都有十分紧密的联系。以DC为基础的免疫治疗正成为慢性HBV感染研究的新方向。既往研究证实慢乙肝患者的DC存在成熟与功能的异常，导致其激活特异性抗病毒免疫反应特别是细胞免疫反应能力低下。因此，恢复慢性乙型肝炎患者DC的功能以增强其激活特异性抗HBV免疫反应能力，则可能达到打破免疫耐受，彻底清除HBV的作用。要保持高水平的持久的抗病毒免疫效应需要以致敏的DC反复刺激机体。为了使DC
[Abstract]:Hepatitis B virus virus (HBV) infection is a worldwide high infection rate, a highly harmful disease: there are about 350 million HBV infections worldwide. The infection rate of hepatitis B virus in China is about 60-70%, among which 15-25% of the infected people will eventually die from HBV related diseases, which makes HBV infection become one of the most serious diseases that harm human beings. At present, the main anti-HBV drugs are interferon and nucleoside analogues lamivudine and so on. But neither of them can eliminate the existence of viral replication template covalently closed circular DNA (cccDNAs). Therefore, the treatment of chronic HBV infection is of great practical significance. Existing studies have found that the low or deficiency of specific cellular immunity plays a crucial role in the pathogenesis of persistent HBV infection. As the most powerful antigen-presenting cells in human body, dendritic cells (DCCs) are the initiators of immune response, which are involved in antigen uptake and presentation. It plays a key role in inducing immune response and is closely related to the pathogenesis and treatment of chronic hepatitis B. Immunotherapy based on DC is becoming a new direction in the study of chronic HBV infection. Previous studies have confirmed that DC in patients with chronic hepatitis B has abnormal maturation and function, resulting in its activation of specific antiviral immune response, especially the ability of cellular immune response is low. Restoring the function of DC in patients with chronic hepatitis B to enhance its ability to activate specific anti HBV immune response may break the immune tolerance. Remove the effect of HBV completely. To maintain a high level of persistent antiviral immune effect, it is necessary for sensitive DC to repeatedly stimulate the body. In order to make DC
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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