乙型肝炎病毒复制型小鼠模型的建立及初步应用

发布时间：2018-01-19 03:02

本文关键词： 高压注射体内转染 HBsAg 免疫组化 HBV 小鼠模型高压注射体内转染病毒复制基因表达　出处：《四川大学》2006年硕士论文　论文类型：学位论文

【摘要】：第一部分利用高压注射体内转染法实现HBsAg在小鼠肝脏中的高效表达目的观察能否应用高压注射体内转染法实现外源DNA在肝脏中高效表达，为嗜肝病毒的分子生物学研究奠定方法学基础。方法将大容量乙肝病毒表面抗原(HBV Surface Antigen，HBsAg)表达质粒pVAX1／S DNA溶液(每只小鼠注射的溶液总量为该小鼠体重的8％，如给体重为20g的小鼠注射的溶液为1.6ml)，，通过尾静脉快速(在5—8秒内)注射入小鼠体内，并采用缓慢注射法为对照。用免疫组化法检测小鼠心、肺、肝、脾、肾组织中HBsAg的表达情况。
[Abstract]:The first part is to realize the high expression of HBsAg in mouse liver by high pressure injection in vivo transfection. Purpose To observe whether high pressure injection in vivo transfection method can be used to realize the high expression of exogenous DNA in the liver, and to lay a methodological foundation for the molecular biology of hepatoviruses. Method Large volume hepatitis B virus surface antigen (HBV Surface Antigen) was used. HBsAg expression plasmid pVAX1/S DNA solution (the total amount of solution injected to each mouse was 8 of the body weight of the mouse, such as 1.6 ml for the 20g mice). The expression of HBsAg in the heart, lung, liver, spleen and kidney of mice was detected by immunohistochemical method after rapid injection of caudal vein (within 5-8 seconds) and slow injection as control.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R512.62

【引证文献】