去卵巢骨质疏松大鼠骨髓间充质干细胞生长分化特点的研究

发布时间：2018-01-19 07:06

本文关键词： 去卵巢骨质疏松大鼠成骨细胞脂肪细胞骨髓间充质干细胞　出处：《四川大学》2005年硕士论文　论文类型：学位论文

【摘要】：绝经后骨质疏松症是一种中老年妇女最常见的全身性骨骼系统疾病。其特点为骨量减少,骨组织微观结构退化,致使骨脆性增加,易于发生骨折。该病严重威胁着绝经后妇女的健康和生命,给家庭和社会带来了巨大的人力和经济负担,已成为当前世界性的主要公共健康问题。目前,关于绝经后骨质疏松症的病因仍不十分清楚。有研究发现绝经后骨质疏松症引起的骨量减少往往与骨髓中的脂肪组织增多相伴行,但是有关其细胞学机制还不清楚。学者认为这种骨量减少与脂肪增多是由于成骨细胞和脂肪细胞的前体细胞-骨髓间充质干细胞(Mesenchymal stem cells,MSCs)向两者分化的交互关系发生了改变。在体内一定条件下,MSCs 可以向成骨细胞、脂肪细胞和软骨细胞等不同的细胞系分化。生理或病理条件下,如果MSCs 生长增殖能力和分化特性发生改变而影响其向成骨细胞系和脂肪细胞系分化的能力,造成骨形成过程和骨髓脂质代谢异常,从而将打破骨形成与骨吸收之间的动态平衡,导致骨质疏松症等疾病。本研究的目的是在建立去卵巢骨质疏松大鼠动物模型的基础上,模拟绝经后骨质疏松的病理变化,探讨体外培养去卵巢骨质疏松大鼠MSCs 细胞增殖能力的改变;MSCs向成骨细胞和脂肪细胞分化能力的改变。以期揭示绝经后骨质疏松症发生的相关细胞分子机制,为绝经后骨质疏松症的诊断与防治提供新思路。选用健康3 月龄和6 月龄雌性Sprague-Dawley(SD)大鼠,采用双侧卵巢切除术,建立去卵巢骨质疏松动物模型。动物实验分成4 组:
[Abstract]:Postmenopausal osteoporosis is one of the most common systemic bone system diseases in middle-aged and elderly women. It is characterized by reduced bone mass and degeneration of bone microstructure, resulting in increased bone brittleness. The disease is a serious threat to the health and life of postmenopausal women and brings a huge human and economic burden to the family and society. It has become a major public health problem worldwide. The etiology of postmenopausal osteoporosis is still unclear. Some studies have found that bone loss caused by postmenopausal osteoporosis is often associated with increased adipose tissue in bone marrow. However, the cytological mechanism is not clear. Scholars believe that this decline in bone mass and fat increase is due to osteoblasts and adipocytes precursor cells-bone marrow mesenchymal stem cells (BMSCs). Mesenchymal stem cells. The interaction between MSCs and osteoblasts has changed. Under certain conditions in vivo, MSCs can be transformed into osteoblasts. Different cell lines such as adipocytes and chondrocytes are differentiated under physiological or pathological conditions. The ability of MSCs to differentiate into osteoblast and adipocyte is affected by the change of growth and proliferation ability and differentiation characteristic of MSCs, and the process of bone formation and lipid metabolism of bone marrow are abnormal. Thus, the dynamic balance between bone formation and bone resorption will be broken, resulting in osteoporosis and other diseases. The purpose of this study is to establish an animal model of ovariectomized osteoporosis in rats. To investigate the proliferation of MSCs cells in ovariectomized osteoporosis rats by simulating the pathological changes of postmenopausal osteoporosis. The change of differentiation ability of MSCs into osteoblasts and adipocytes in order to reveal the cellular molecular mechanism of postmenopausal osteoporosis and to provide a new idea for the diagnosis and prevention of postmenopausal osteoporosis. Female Sprague-Dawley SD rats aged 3 and 6 months were selected and bilateral ovariectomy was used to establish ovariectomized osteoporosis animal model.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R361

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