抗癌中草药及其与DNA相互作用的电化学研究

发布时间：2018-01-21 05:18

本文关键词： 抗癌中草药大黄素芹菜素电化学 DNA　出处：《郑州大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目前，中药尤其抗癌中草药引起了许多制药机构的高度重视，因为研究者开始意识到中草药对于药物发展来说是一个巨大的来源，，而且中药的毒性比较低，很多没有副作用。据知，现在对中草药的研究多集中在提纯、分离方面，在其性质及其抗癌机理的研究仍然很欠缺。本论文研究了抗癌中草药大黄素和芹菜素的电化学性质，建立了两种药物的电化学分析方法，并运用电化学和光谱学手段研究了抗癌中草药大黄素和芹菜素与DNA的相互作用，初步讨论了大黄素通过插入到DNA的双螺旋链中来抑制DNA的合成，从而达到抗肿瘤的效果；芹菜素与DNA在该实验条件下不发生作用，显示了芹菜素低毒的特性。本论文主要包括以下几个内容： 1．研究了抗癌药物大黄素在玻碳电极上的电化学行为。在pH 7.2的NH_3-NH_4Cl(50％无水乙醇)缓冲溶液中，大黄素有三个均受吸附控制的氧化和还原峰，还原峰的峰电位为-0.688V(P1)，两个氧化峰的峰电位为-0.628V(P2)和-0.235V(P3)。其中P3峰的峰电流与大黄素的浓度在8.9×10~(-8)M～7.8×10~(-6)M范围内有良好的线性关系，检出限为7.8×10~(-9)M，对三黄片实际样品中大黄素含量的测定，不用预分离其它蒽醌类化合物，结果令人满意。 2．利用多种电化学手段研究了抗癌中草药芹菜素在B-R(50％无水乙醇，pH 9.0)缓冲底液中的电化学性质。循环伏安图谱表明其电极过程是一扩散控制为主的氧化反应，呈现了一个不可逆的氧化峰。文章分别运用Laviron理论计算了芹菜素在玻碳电极上的动力学参数，并以该氧化峰为研究对象建立了芹菜素的电化学分析方法。在所选实验条件下，芹菜素浓度在5.0×10~(-6)M～9.0×10~(-5)M范围内与峰电流存在良好的线性关系，检测限为1.5×10~(-6)M，并对密蒙花实际样品的芹菜素进行了含量测定，结果令人满意。并用差示脉冲伏安法和紫外可见方法研究了芹菜素与DNA的相互作用，结果表明在该实验条件下，芹菜素与DNA不发生相互作用，从而显示了芹菜素低毒的一面。 3．在pH 5.5，0.05 mol／LNH_4Cl-HCl(50％无水乙醇)缓冲溶液中，利用差示脉冲技术在裸玻碳电极和DNA修饰电极上研究了抗癌中草药大黄素与DNA的相互作用，结果表明随着DNA的加入，大黄素的峰电位发生了明显正移且峰电流降低，同时紫外可见光谱也表现出其440nm的吸怛光度值降低，最大吸收红移，而且在400nm和475nm处出现两个等吸收点，所有这些表明大黄素以其接近平面的结构插入到DNA的双螺旋结构中，从而抑制DNA的合成，达到抗肿瘤效果。另外，在最优化条件下，大黄素峰电流的降低与DNA的浓度在一定范围内存在良好的线性关系，以此进行了DNA样品回收率的测定，结果令人满意。
[Abstract]:At present, Chinese medicine, especially anti-cancer Chinese medicine, has attracted great attention from many pharmaceutical organizations, because researchers have begun to realize that Chinese medicine is a huge source of drug development, and the toxicity of traditional Chinese medicine is relatively low. Many have no side effects. It is known that the current research on Chinese herbal medicine is focused on purification and separation. In this paper, the electrochemical properties of emodin and apigenin were studied, and the electrochemical analysis method of two kinds of drugs was established. The interaction of emodin and apigenin with DNA was studied by electrochemical and spectroscopic methods. The inhibition of DNA synthesis by emodin inserted into the double helix chain of DNA was discussed preliminarily. In order to achieve the effect of anti-tumor; Apigenin and DNA do not interact under this experimental condition, which shows the low toxicity of apigenin. This thesis mainly includes the following contents: 1. The electrochemical behavior of the anticancer drug emodin on glassy carbon electrode was studied in the pH 7.2 NH3-NH4Cl-50% anhydrous ethanol buffer solution. Emodin had three oxidation and reduction peaks controlled by adsorption, and the peak potential of the reduction peak was -0.688V ~ (-1). The peak potentials of the two oxidation peaks were -0.628 V ~ (2) and -0.235 V ~ (3) P _ (3). The peak current of P3 peak and the concentration of emodin were 8.9 脳 10 ~ (-8)). There is a good linear relationship in the range of 7.8 脳 10 ~ (-1) ~ (-6) ~ (-1). The detection limit of emodin in Sanhuang tablets was 7.8 脳 10 ~ (10) ~ (-9) 渭 m. The determination of emodin in Sanhuang tablets was satisfactory without the need of preseparation of other anthraquinones. 2. 50% anhydrous ethanol with apigenin, an anticancer herb, was studied by various electrochemical methods. The electrochemical properties in buffer solution were determined by pH 9.0. The cyclic voltammetry showed that the electrode process was mainly a diffusion-controlled oxidation reaction. There is an irreversible oxidation peak. The kinetic parameters of apigenin on glassy carbon electrode were calculated by Laviron theory. The electrochemical analysis method of apigenin was established based on the oxidation peak. There was a good linear relationship between the concentration of apigenin and peak current in the range of 5.0 脳 10 ~ (-1) -6 ~ (-1) M ~ (9) 脳 10 ~ (10) ~ (-5) ~ (5) M, and the detection limit was 1.5 脳 10 ~ (10) ~ (-6) 渭 m. The content of apigenin in the actual sample was determined with satisfactory results. The interaction between apigenin and DNA was studied by differential pulse voltammetry and UV-vis. The results showed that there was no interaction between apigenin and DNA under the experimental conditions, which showed the low toxicity of apigenin. 3. In the buffer solution of pH 5.5 ~ (0.05) mol / L ~ (NH _ 4NH _ 4Cl-HCln _ (50)% anhydrous alcohol). The interaction between emodin and DNA was studied on bare glassy carbon electrode and DNA modified electrode by differential pulse technique. The results showed that the interaction between emodin and DNA was increased with the addition of DNA. The peak potential of emodin showed a significant positive shift and peak current decreased, while the UV-Vis spectrum also showed a decrease in absorbance value of 440 nm, and the maximum absorption red shift. Moreover, there are two isoabsorption points at 400nm and 475nm, all of which indicate that emodin is inserted into the double helix structure of DNA with its near plane structure, thus inhibiting the synthesis of DNA. In addition, under the optimal conditions, there is a good linear relationship between the peak current of emodin and the concentration of DNA in a certain range, so the recovery rate of DNA samples is determined. The result is satisfactory.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R285;R284

【引证文献】