结核菌感染巨噬细胞钙离子通道表达的研究

发布时间：2018-01-22 02:05

本文关键词： 钙通道巨噬细胞结核分枝杆菌　出处：《中国病原生物学杂志》2014年11期 　论文类型：期刊论文

【摘要】：目的研究结核分枝杆菌(MTB)感染的小鼠腹腔巨噬细胞L-型钙通道(L-type calcium channel,LTCC)的mRNA表达水平,并探讨其免疫学意义。方法结核分枝杆菌强毒株H37Rv、弱毒株H37Ra、卡介菌(简称BCG)分别感染小鼠24h后,腹腔灌洗收获巨噬细胞,用逆转录-聚合酶链反应(RT-PCR)半定量技术检测巨噬细胞LTCC mRNA的表达情况,并选择正常小鼠和金黄色葡萄球菌(金葡菌)感染小鼠作对照。结果巨噬细胞LTCC mRNA表达水平(相对含量)MTB H37Rv组为0.42±0.14),H37Ra组为0.35±0.09,BCG组为0.32±0.10,与正常未感染组0.20±0.08比较差异有统计学意义(P0.01或P0.05);H37Rv、H37Ra感染组与金葡菌感染组0.24±0.07比较差异有统计学意义(P0.01或P0.05);BCG感染组与金葡菌感染组之间差异无统计学意义(P0.05);金葡菌感染组与正常未感染组之间差异也无统计学意义(P0.05);BCG感染组、H37Ra感染组和H37Rv感染组间差异无统计学意义(P0.05);不同抗菌谱的金葡菌感染组间差异也无统计学意义(P0.05)。结论 MTB感染小鼠后巨噬细胞LTCC mRNA表达量显著增加,提示LTCC在MTB的致病机制和机体的免疫保护机制中均发挥了一定的作用。
[Abstract]:Objective to study the Mycobacterium tuberculosis (MTB) in mouse peritoneal macrophages of L- type calcium channel infection (L-type calcium channel, LTCC) mRNA expression level, and to study its immunological significance. Mycobacterium tuberculosis virulent strain H37Rv, strain H37Ra, BCG (BCG) respectively in mice infected with 24h after harvest, peritoneal lavage macrophages and by using reverse transcription polymerase chain reaction (RT-PCR) to detect the expression of macrophage LTCC mRNA semi quantitative technique, and the selection of normal mice and Staphylococcus aureus (S.aureus) infection were used as control. Results the macrophage LTCC expression level of mRNA (relative content) MTB H37Rv group is 0.42 + 0.14), H37Ra group 0.35 + 0.09, BCG group is 0.32 + 0.10, and the normal uninfected group was statistically significant difference between the 0.20 + 0.08 (P0.01 or P0.05); H37Rv, H37Ra infection group and S.aureus infection group was statistically significant difference (P0.0 0.24 + 0.07 1 or P0.05); there was no significant difference between BCG infection group and S.aureus infection group (P0.05); non infection group there was no significant difference between normal group and Staphylococcus aureus infection (P0.05); BCG infection group, H37Ra infection group and H37Rv infection group showed no significant difference (P0.05); different the antibacterial spectrum of Staphylococcus aureus infection groups there was no significant difference (P0.05). LTCC mRNA expression was significantly increased in macrophages of mice infected with the MTB conclusion, suggesting that the immune protective mechanism of LTCC in the pathogenesis of MTB and the body have played a certain role.

【作者单位】：河北北方学院;解放军第309医院全军结核病研究所全军结核病防治重点实验室;
【基金】：国家重大传染病防治科技重大专项基金项目(No.2012ZX10003008002)
【分类号】：R378.911
【正文快照】： ***结核病是严重危害人类健康的主要呼吸道传染病。近年来,发病率虽然有所下降,但是随着耐药菌株出现、HIV复合感染等因素影响,使结核病的治疗面临着巨大的挑战[1]。最近有研究表明,Ca2+内流增加或钙池Ca2+释放,可以影响胞内钙信号转导,引起抗炎蛋白基因表达和细胞因子的释放,

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