福建地区HCV基因型及Core蛋白的差异研究

发布时间：2018-01-25 11:40

本文关键词： 丙型肝炎病毒 Core蛋白基因型　出处：《福建医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 丙型肝炎病毒为单股正链RNA病毒,基因组全长约9.4kb。丙型肝炎进展迅速且后果严重,极易转变为肝硬化和肝癌,是危害人类健康的重要传染病之一。HCV分为6个基因型,各型的地域分布,疾病表现以及对干扰素的治疗反应有很大差别,对HCV基因型及致病相关基因的结构和功能的差别研究有助于判断疾病的预后以及选择合适的治疗方案。本研究旨在了解福建地区HCV基因型,并对Core蛋白(HCV最重要的致病蛋白之一)进行氨基酸差异分析。本研究的第一部分为:巢式RT-PCR扩增血清样品HCV core基因部分区段(-12nt +343nt),克隆并测序;以Clustalw软件对所克隆片段进行系统进化树分析并分型,了解福建地区的HCV基因型分布。结果表明在102份福建地区HCV抗体阳性血清中,HCV检出率为41.2%(共42份),系统进化树分析提示共有1b、2a、3b、6a四个不同的基因型,以6a和1b基因型为主。本研究的第二部分为:RT-PCR扩增并克隆上述四个基因型的HCV全长core基因并比较各基因型Core蛋白的氨基酸差别。结果显示各型HCV Core蛋白共有33个氨基酸差别,主要集中4aa-21aa,68aa-78aa,106aa-121aa,139aa-163aa,182aa-191aa五个区域,不同基因型HCV在这些区域氨基酸的差别可能会导致Core蛋白的功能差异并影响其致病性。
[Abstract]:Hepatitis C virus (HCV) is a single-stranded positive strand RNA virus with a genome length of about 9.4 kb.Hepatitis C develops rapidly and has serious consequences. It is easy to be transformed into liver cirrhosis and liver cancer. HCV is one of the most important infectious diseases that endanger human health. HCV is divided into 6 genotypes. The regional distribution of each type, disease manifestation and therapeutic response to interferon are very different. The study on the structure and function of HCV genotypes and pathogenicity related genes is helpful to determine the prognosis of the disease and to select the appropriate treatment plan. This study aims to understand the genotype of HCV in Fujian province. Amino acid difference analysis was carried out for one of the most important pathogenic proteins of Core protein. The first part of this study was: nested RT-PCR amplification of the partial region of HCV core gene from serum samples, cloning and sequencing; The phylogenetic tree analysis and typing of the cloned fragments were carried out with Clustalw software to understand the distribution of HCV genotypes in Fujian. The results showed that 102 sera were positive for HCV antibodies in Fujian. The detection rate of HCV was 41.2%. Phylogenetic tree analysis showed that there were four different genotypes (6a and 1b). The second part of this study is as follows:. The HCV full-length core gene of the four genotypes was amplified and cloned by RT-PCR, and the amino acid differences of the Core proteins of the four genotypes were compared. There are 33 amino acid differences in Core protein. It is mainly concentrated in the five regions of 4aa-21aan 68aa-78aan 106aa-121aan 139aa-163aanjiao 182aa-191aa. The difference of amino acids between different genotypes of HCV in these regions may lead to the functional difference of Core protein and affect its pathogenicity.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

【共引文献】