髓源抑制性细胞在日本血吸虫感染小鼠脾脏及外周血富集的研究

发布时间：2018-01-25 22:47

本文关键词： 日本血吸虫髓源抑制性细胞免疫逃避流式细胞术　出处：《中国寄生虫学与寄生虫病杂志》2014年01期 　论文类型：期刊论文

【摘要】：目的分析日本血吸虫尾蚴感染小鼠体内髓源抑制性细胞(myeloid-derived suppressor cells,MDSC)的富集情况,初步探索其在抗血吸虫感染免疫中的作用。方法日本血吸虫尾蚴用腹部贴片法感染C57BL/6小鼠(20条/鼠)24只。感染后1、2、6和8周采集小鼠(各6只)外周血,感染6、8周组小鼠脱颈处死后取脾组织,制备单细胞悬液。各时段同时设健康对照组(各6只)。通过流式细胞术检测小鼠脾组织和外周血中的Gr-1+细胞、CD11b+细胞及MDSC比例。通过CD4+T细胞增殖抑制试验验证感染小鼠Gr-1+粒细胞的功能。结果感染后6周和8周组小鼠外周血MDSC、Gr-1+细胞、CD11b+细胞分别约占总单核细胞(MNC)的38.2%~57.8%和47.1%~77.6%,28.9%~44.6%和40.4%~72.9%,36.0%~48.1%和40.3%~68.3%,显著高于健康对照组(15.1%~20.4%,8.4%~17.3%,9.8%~22.6%)、感染后1周(16.2%~19.8%,13.0%~16.8%,17.6%~19.4%)及2周组(19.8%~29.5%,17.2%~22.2%和20.9%~33.3%)(P0.01)。而感染后1周、2周组与健康对照组相比,差异无统计学意义(P0.05)。脾组织与外周血中的MDSC、Gr-1+细胞、CD11b+细胞变化趋势一致。此外,从感染小鼠脾组织分离到的Gr-1+细胞显著抑制刀豆球蛋白诱导的健康小鼠的CD4+T细胞增殖能力。结论日本血吸虫感染可诱导小鼠体内MDSC富集,且感染Gr-1+细胞可抑制正常CD4+T细胞增殖。
[Abstract]:Objective to analyze the enrichment of myeloid inhibitory cells (MDSCs) in mice infected with Schistosoma japonicum cercariae. Methods Schistosoma japonicum cercariae were infected with 20 C57BL / 6 mice by abdominal patch method. Peripheral blood was collected from each of the mice at 6 and 8 weeks, and splenic tissue was taken from the mice in the 6- week group. Single cell suspension was prepared. Gr-1 cells in spleen and peripheral blood of mice were detected by flow cytometry. The ratio of CD11b cells to MDSC. The function of Gr-1 granulocyte in infected mice was tested by CD4 T cell proliferation inhibition test. Results the peripheral blood MDSC of the infected mice was 6 weeks and 8 weeks after infection. The percentage of CD11b cells in Gr-1 cells was 57.8% and 77.6% in total monocytes, respectively. 28.9% 44.6% and 40.4% 72.9% were 48.1% and 40.3%, significantly higher than that of the healthy control group, 15.1% and 20.4%. 8.4The value of 17.3m / 9.8B is 22.6m, 16.2%, 19.813.0% and 16.8%, one week after infection. (17.6%) and 2 weeks group (19.829.5) and 17.2% and 20.9% and 33.33.3P 0.01%, respectively, and one week after infection. There was no significant difference between the 2-week group and the healthy control group (P 0.05). The change trend of CD11b cells in the spleen tissue and the peripheral blood of MDSC-Gr-1 cells was the same. In addition, the change of CD11b cells in the spleen tissue was the same as that in the peripheral blood. Gr-1 cells isolated from spleen tissue of infected mice significantly inhibited CD4 in healthy mice induced by concanavalin. Conclusion Schistosoma japonicum infection can induce MDSC enrichment in mice. Infection of Gr-1 cells inhibited the proliferation of normal CD4 T cells.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织疟疾、血吸虫病和丝虫病合作中心;
【基金】：国家自然科学基金(No.81371841,81371842,30872212) 国家传染病科技重大专项(No.2012ZX10004-201)~~
【分类号】：R392
【正文快照】： 近年来,血吸虫免疫调节机制研究取得较大进展[1]。血吸虫感染宿主随着感染时间延长逐渐表现出免疫应答总体下调现象,不仅对血吸虫特异性抗原刺激表现为低应答,对其他抗原(或免疫原)刺激的应答也明显下降[2]。研究证实,许多调节性细胞参与了宿主抗血吸虫免疫反应,如Th1细胞、Th

【参考文献】