不同裂解工艺制备的甲型H1N1流感病毒裂解疫苗的透射电子显微镜观察

发布时间：2018-01-30 20:45

本文关键词： 流感病毒A型 HN亚型疫苗裂解工艺透射电子显微镜　出处：《中国生物制品学杂志》2014年03期 　论文类型：期刊论文

【摘要】：目的透射电子显微镜观察不同工艺制备的甲型H1N1流感病毒裂解疫苗形态,筛选最佳裂解工艺,为疫苗的大规模生产提供参考依据。方法应用透射电镜对4种不同裂解工艺制备的甲型H1N1流感病毒疫苗进行形态观察,采用中和试验分析其免疫原性,异常毒性试验分析其安全性。结果透射电镜观察显示4种样品均无完整病毒,样品1裂解程度最大,囊膜破裂,多数为病毒碎片;样品2大多数囊膜未破损,缺少纤突;样品3较完整的病毒颗粒略小(约30~40 nm),纤突清晰;样品4大多数病毒颗粒保留着纤突,但纤突已不整齐清晰。样品2组小鼠中和抗体滴度最低,而样品1、3、4组较高,与样品2组相比,差异明显,其中以样品3组最高;样品2组小鼠、豚鼠体重增加不明显,而样品1、3、4组小鼠、豚鼠体重增加明显,与样品2组相比差异显著,其中以样品3组最明显。结论电镜观察可直观反映疫苗裂解程度;3种裂解工艺均可用于疫苗生产,其中样品3具有较好的免疫原性和安全性,其工艺可作为最佳裂解工艺用于后续疫苗生产。
[Abstract]:Objective to observe the morphology of influenza A (H1N1) virus lytic vaccine prepared by transmission electron microscope (TEM) and to screen the best lysis technique. Methods using transmission electron microscope (TEM) to observe the morphology of four kinds of influenza A (H1N1) vaccine prepared by different pyrolysis process, and to analyze its immunogenicity by neutralization test. Results the results of transmission electron microscopy showed that none of the four samples had intact virus, and sample 1 had the largest cleavage degree and the rupture of the capsule membrane, most of which were virus fragments. Most of the capsule in sample 2 were not damaged and lack of fibrous process. The virus particles in sample 3 were slightly smaller (about 30 ~ 40 nm) and the fibrils were clear. The titer of neutralizing antibody in group 2 was the lowest, while the titer of neutralizing antibody in group 1 was higher than that in group 2, which was significantly different from that in group 2. Among them, the sample group 3 was the highest. The weight gain of guinea pigs in group 2 was not significant, but that in group 1 was significantly higher than that in group 2. Conclusion Electron microscope observation can directly reflect the degree of vaccine cleavage. All of the three lytic processes can be used in vaccine production, among which sample 3 has better immunogenicity and safety, and its process can be used as the best lysis process for subsequent vaccine production.
【作者单位】：中国人民解放军军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室;长春生物制品研究所有限责任公司;
【分类号】：R373.13
【正文快照】： 近年来,市场上的流感疫苗均为纯化的灭活疫苗,包括全病毒灭活疫苗、裂解疫苗及亚单位疫苗,其中裂解疫苗因其免疫效果好、副作用小而被广泛应用[1]。该疫苗是选用适当裂解剂和裂解条件对流感病毒进行裂解,经纯化除杂等步骤后制备,病毒裂解不完全或裂解过度均将直接影响疫苗质量

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