小鼠骨髓及脾脏髓源抑制细胞Notch分子表达分析

发布时间：2018-02-04 04:16

本文关键词： Notch受体 Notch配体 Notch信号髓源抑制细胞　出处：《现代免疫学》2014年04期 　论文类型：期刊论文

【摘要】：为研究Notch信号途径相关分子在小鼠髓源抑制细胞(MDSC)中表达水平,以CD11b+和Gr-1+为表面标记,采用流式细胞术分选出小鼠骨髓与脾脏MDSC,进而采用定量RT-PCR技术检测Notch 4种受体、5种配体及其下游靶基因Hes-1的mRNA表达水平。结果发现,骨髓中CD11b+Gr-1+细胞(MDSC)比例远高于脾脏,达总细胞的46%,而脾脏MDSC仅占总细胞的4.4%左右。骨髓与脾脏MDSC表达Notch四种受体分子,四种受体分子在两种MDSC中表达水平未见明显差异(P0.05)。骨髓MDSC Dll4表达水平明显高于脾脏MDSC(P0.001),而Jagged1、Jagged2和Dll1三种配体分子在来源于两种淋巴器官的MDSC中表达水平未见明显差异(P0.05),二者均不表达Dll3。与骨髓MDSC相比,脾脏MDSC Hes-1mRNA表达明显增高(P0.01)。结果提示Notch信号途径可能在MDSC成熟与迁移中发挥一定的作用。
[Abstract]:In order to study the expression of Notch signaling pathway related molecules in mouse myeloid suppressor cells, CD11b and Gr-1 were used as surface markers. The bone marrow and spleen of mice were separated by flow cytometry, and four kinds of Notch receptors were detected by quantitative RT-PCR. The results showed that the proportion of CD11b Gr-1 cells in bone marrow was much higher than that in spleen, reaching 46% of total cells. The spleen MDSC accounted for only about 4.4% of the total cells. Notch receptor molecules were expressed in bone marrow and spleen MDSC. There was no significant difference in the expression level of four receptor molecules in two kinds of MDSC. The expression of MDSC Dll4 in bone marrow was significantly higher than that in spleen (P 0.001). There was no significant difference in the expression of Jagged2 and Dll1 ligands in MDSC from the two lymphoid organs (P 0.05). Neither of them expressed Dll3.Compared with bone marrow MDSC. The expression of MDSC Hes-1mRNA in spleen was significantly increased (P 0.01). The results suggested that the Notch signaling pathway might play a role in the maturation and migration of MDSC.
【作者单位】：江苏大学附属医院检验科、中心实验室镇江市医学免疫学重点实验室;江苏大学基础医学与医学技术学院病原生物学系;
【基金】：国家自然科学基金(81172871;81373217)
【分类号】：R392.11
【正文快照】： 髓源抑制细胞(myeloid-derived suppressorcells,MDSC)是一群来源于骨髓的异质性细胞群体,主要由髓系祖细胞(myeloid-cell progenitors)和未成熟髓细胞(immature myeloid cells)组成,未成熟髓细胞即髓细胞前体(precursors of myeloidcells)正常情况下可进一步分化为成熟的粒细

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