血管内皮抑制因子angiostatin和thalidomide对淋巴管内皮细胞生成的影响

发布时间：2018-02-05 16:55

本文关键词： 血管抑制素反应停淋巴管内皮细胞血管抑制因子凋亡　出处：《青岛大学》2005年硕士论文　论文类型：学位论文

【摘要】：目的探讨血管生成抑制素(angiostatin)和反应停(thalidomide)对淋巴管内皮细胞增殖和游走的影响及作用机制。旨在找到安全、有效、实用的淋巴管生成抑制剂。材料和方法实验所用的淋巴管内皮细胞取自猪的胸导管。1、抑制实验:本实验分为两大组:angiostatin组和thalidomide组。每大组分别采用划线法和MTT法观察两种因子对淋巴管内皮细胞增殖和游走的影响。(1)划线法:angiostatin和thalidomide组分别包括各自的对照组和3种浓度的实验组。(2)MTT法:angiostatin组包括对照组和4种浓度的实验组;thalidomide组包括对照组和5种浓度的实验组。2、细胞凋亡实验:(1)Hoechst染色法。(2)流式细胞仪检测法:配置不同浓度的angiostatin和thalidomide作用于淋巴管内皮细胞检测细胞的凋亡率。3电镜观察。结果 1、划线法:与对照组相比,当angiostatin和thalidomide的浓度达到0.5μg/ml时,两者对淋巴管内皮细胞的增殖和游走都有明显的抑制作用(P0.01)。2、MTT法:当angiostatin和thalidomide的浓度分别达到0.75μg/ml和0.5μg/ml时,两者对内皮细胞的增殖具有显著的抑制作用(P0.01)。3、凋亡实验:(1)Hoechst染色证实,经angiostatin和thalidomide处理后的淋巴管内皮细胞,可观察到其核周围有凋亡小体。(2)流式细胞仪检测显示出大量亚二倍体细胞核型峰(凋亡峰),二倍体细胞峰减少。结论 1、angiostatin和thalidomide对淋巴管内皮细胞的增殖和游走具有明显的抑制作用,且有剂量依赖性。2、angiostatin和thalidomide具有促进细胞凋亡的作用。
[Abstract]:Objective to study angiostatin (angiostatin) and thalidomide in response to angiogenesis. The effect and mechanism on proliferation and migration of lymphatic endothelial cells. Effective, practical lymphangiogenesis inhibitor. Materials and methods the lymphatic endothelial cells used in the experiment were taken from the thoracic ducts of pigs. Inhibition experiment: this experiment is divided into two groups:. In angiostatin group and thalidomide group, the effects of two factors on the proliferation and migration of lymphatic endothelial cells were observed by underlined method and MTT method respectively. 1) the two groups: the control group and the thalidomide group included their respective control groups and three different concentrations of experimental groups (n = 2). MTT method: angiostatin group included control group and four experimental groups of different concentrations. The thalidomide group included control group and experimental group of 5 different concentrations. Apoptosis experiment: 1: 1 Hoechst staining. Flow cytometry: the apoptotic rate of lymphatic endothelial cells was observed by electron microscope with different concentrations of angiostatin and thalidomide. Results 1. Line method: compared with the control group, when the concentration of angiostatin and thalidomide reached 0.5 渭 g / ml. Both of them had obvious inhibitory effects on proliferation and migration of lymphatic endothelial cells. MTT method: when the concentration of angiostatin and thalidomide reached 0.75 渭 g / ml and 0.5 渭 g / ml, respectively. Both of them had a significant inhibitory effect on endothelial cell proliferation. The apoptosis experiment was confirmed by Hoechst staining. The lymphatic endothelial cells were treated with angiostatin and thalidomide. It was observed that there were apoptotic corpuscles around its nucleus. Flow cytometry showed that there were a large number of nuclear peaks (apoptosis peak and diploid cell peak decreased) in hypodiploid cells. Conclusion 1 angiostatin and thalidomide can inhibit the proliferation and migration of lymphatic endothelial cells in a dose-dependent manner. Angiostatin and thalidomide can promote apoptosis.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329

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