小鼠胃、胰腺中内分泌细胞的形态学研究

发布时间：2018-02-05 21:37

本文关键词： 小鼠胃体部 ECL 细胞内分泌细胞主细胞壁细胞透射电镜　出处：《山西医科大学》2005年硕士论文　论文类型：学位论文

【摘要】：目的:观察小鼠胃体部粘膜内分泌细胞的超微结构,及内分泌细胞与胃底腺主细胞、壁细胞等细胞的相互关系。方法:利用透射电镜技术,观察成年小鼠胃体部粘膜内分泌细胞和主细胞、壁细胞的超微结构及位置关系。结果:电镜下,根据所观察的内分泌细胞中颗粒超微结构的不同特点,可将这些细胞分为I、II、III 型内分泌细胞。 I 型内分泌细胞的胞质内含有两类颗粒,一类为泡状,内含一个偏心的电子密度高的核心,颗粒界膜与核心间有很宽的空晕;另一类颗粒中有圆形、均质或不均质的核心,有一完整界膜,颗粒界膜与核心之间有清晰的窄隙。除分泌颗粒外,胞质中还可见大量的胞质小泡。II 型内分泌细胞的分泌颗粒中有电子密度较低、均质状的核心,核心与界膜之间有狭窄的空晕。III 型内分泌细胞的颗粒可分为两类,一类颗粒中有一个电子密度较高、均质状的核心,核心与界膜之间有狭窄的间隙或无间隙,有时在均质的核心中还可见一电子密度更高的小核心;另一类颗粒中有一个电子密度较低、均质或不均质状的核心,核心与界膜之间形成较宽的间隙。三种类型的内分泌细胞均可见与主细胞及壁细胞紧密相邻,内分泌细胞膜的局部呈“Ω”型凹陷。结论:基本确认本实验所见的I 型内分泌细胞即为肠嗜铬样细胞(Enterochromaffin like cell ,简称ECL 细胞),多见该细胞与主细胞、壁细胞紧密相邻,推测ECL 细胞除对壁细胞泌酸有影响外,可能在功能上与主细胞之间也存在密切关系。胃体部内分泌细胞除以经典内分泌方式释放激素外,也可以旁分泌方式对周围细胞产生影响。
[Abstract]:Aim: to observe the ultrastructure of endocrine cells in the gastric mucosa of mice and the relationship between endocrine cells and main and parietal cells of gastric fundus. Methods: the ultrastructure and location of endocrine cells and main cells and parietal cells in gastric mucosa of adult mice were observed by transmission electron microscopy. Results: according to the observed ultrastructural characteristics of endocrine cells, these cells can be classified into type III endocrine cells. There are two kinds of granules in the cytoplasm of type I endocrine cells, one is bubbly, contains an eccentric core with high electron density, and there is a very wide hollow halo between the granular boundary membrane and the core. In another kind of particles, there is a round, homogeneous or uneven core, a complete boundary membrane, a clear narrow gap between the intergranular membrane and the core, with the exception of secretory granules. In the cytoplasm, a large number of secretory granules of cytoplasmic vesicles. II endocrine cells have low electron density and homogeneous core. There are two types of narrow hollow halo. III endocrine cells between the core and the boundary membrane. In one kind of granules, there is a high electron density and homogeneous core. There is a narrow gap or no gap between the core and the boundary membrane, and sometimes a small nucleus with higher electron density can be seen in the homogeneous core. In the other kind of granules, there is a low electron density, homogeneous or heterogeneous core, with a wide gap between the core and the boundary membrane. The three types of endocrine cells are closely adjacent to the main cells and parietal cells. The local area of endocrine cell membrane is "惟" type depression. Conclusion: the type I endocrine cells identified in this study are enterochromaffin like cell (ECL cells). Most of the cells were adjacent to the main cells and parietal cells. It was speculated that ECL cells had an effect on the secretion of acid in parietal cells. It is possible that there is a close relationship between the function of the endocrine cells and the main cells. In addition to releasing hormones in the classical endocrine mode, the endocrine cells in the body of the stomach can also influence the peripheral cells in the paracrine way.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329

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