恶性疟原虫抗原对健康人外周血T细胞免疫功能的影响

发布时间：2018-02-06 01:08

本文关键词： 恶性疟原虫抗原免疫功能 T细胞 IL- IFN-γ　出处：《中国血吸虫病防治杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的探讨恶性疟原虫抗原对健康人外周血T淋巴细胞免疫功能的影响。方法健康成人外周血单个核细胞(PBMC)体外分别用恶性疟原虫(P.f)抗原(5μg/ml)和正常红细胞(nRBC)抗原(5μg/ml)进行刺激,同时给予IL-2维持细胞增殖,另外设立只加IL-2的阴性对照组进行培养。培养12 d时,刺激组细胞再用对应的抗原刺激20 h,用流式细胞仪检测T淋巴细胞亚群分泌IL-4和IFN-γ的情况,并用羧基荧光素乙酰乙酸琥珀酰亚胺酯(Carboxyfluorescein diacetate,succinimidyl ester,CFSE)标记法检测T细胞增殖反应。结果健康人PBMC经P.f抗原刺激扩增后CD4+T细胞的增殖指数(PI)明显高于nRBC抗原刺激组和阴性对照组(P均0.05),但3组γδT的PI差异无统计学意义(P0.05)。P.f抗原组分泌IL-4的CD4+T细胞百分率明显高于nRBC抗原组和阴性对照组(P均0.05),但3组分泌IFN-γ的CD4+T细胞百分率差异无统计学意义(P0.05)。结论 P.f抗原在体外可刺激健康人外周血CD4+T细胞增殖活化,后者通过优先分泌IL-4而发挥免疫调节作用。
[Abstract]:Objective to investigate the effect of Plasmodium falciparum antigen on the immune function of peripheral blood T lymphocytes in healthy adults. 5 渭 g / ml) and 5 渭 g / ml of normal RBC antigen were stimulated. At the same time, IL-2 was given to maintain cell proliferation, and a negative control group with only IL-2 was set up for culture. After 12 days of culture, the cells in the stimulation group were stimulated with the corresponding antigen for 20 h. The secretion of IL-4 and IFN- 纬 by T lymphocyte subsets was detected by flow cytometry. Carboxyfluorescein diacetate succinimidyl ester was used as carboxyl fluorescein acetoacetate succinimidyl ester. Results the proliferative index (Pi) of CD4 T cells stimulated by P.F antigen was determined by CFSE labeling method. It was significantly higher than that of nRBC antigen stimulation group and negative control group (P 0.05). However, the Pi difference of 纬未 T in the three groups was not statistically significant. The percentage of CD4 T cells secreting IL-4 in the P0.05 + P.F antigen group was significantly higher than that in the nRBC antigen group and the negative control group (P < 0.05). P = 0.05). However, there was no significant difference in the percentage of CD4 T cells secreting IFN- 纬 among the three groups. Conclusion P.F antigen can stimulate the proliferation and activation of CD4 T cells in healthy human peripheral blood in vitro. The latter plays an immunomodulatory role by preferentially secreting IL-4.
【作者单位】：蚌埠医学院病原生物学教研室、安徽省感染与免疫重点实验室;
【基金】：蚌埠医学院研究生科研创新计划资助项目(Byy.cx1304) 安徽省教育厅自然科学研究重点项目(KJ2012.A.200)
【分类号】：R392

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