声触诊组织定量技术评估兔慢性肾病肾纤维化的实验研究

发布时间：2018-02-08 14:06

本文关键词： 肾疾病慢性病纤维化超声检查多普勒彩色声触诊组织量化技术疾病模型动物兔　出处：《中国医学影像学杂志》2014年09期 　论文类型：期刊论文

【摘要】：目的建立新西兰兔慢性肾病模型,应用声触诊组织定量(VTQ)技术监测兔慢性肾病的发展,为VTQ评估慢性肾病肾纤维化提供实验依据。材料与方法选取38只健康新西兰大白兔,其中32只每天经耳缘静脉注射阳离子牛血清白蛋白(C-BSA)诱导慢性肾病模型,另外6只未注射C-BSA设为对照组。于0周、2周、4周、6周、8周采用VTQ测量兔肾皮质硬度,随后解剖兔,评估肾脏的病理变化,并分析VTQ参数与病理参数的相关性。结果兔肾皮质0周、2周、4周、6周、8周的VTQ值分别为(1.68±0.25)m/s、(1.70±0.31)m/s、(1.87±0.35)m/s、(2.19±0.31)m/s、(2.46±0.46)m/s,6周VTQ值与4周比较差异有统计学意义(P0.05),8周VTQ值与4周、6周比较差异有统计学意义(P0.05)。4周、6周、8周肾小球硬化指数分别为0.81±0.40、1.43±0.46、2.15±0.46,肾间质胶原纤维面积分别为(14.29±4.62)%、(26.28±11.06)%、(42.37±10.09)%,均明显高于0周、2周,且纤维化随病程进展逐渐增高,差异有统计学意义(P0.05)。肾皮质VTQ与肾小球硬化指数、肾间质胶原纤维面积呈正相关(r=0.663、0.652,P0.05)。结论 VTQ可以检测到兔慢性肾病发展过程中肾皮质硬度增高,与肾纤维化呈正相关,提示VTQ可以成为评价肾纤维化的重要方法。
[Abstract]:Objective to establish a new Zealand rabbit model of chronic nephropathy and to monitor the development of chronic nephropathy by using the technique of acoustic palpation tissue quantitative quantitation (VTQQ) in order to provide experimental basis for the assessment of renal fibrosis in chronic nephropathy by VTQ. Materials and methods 38 healthy New Zealand white rabbits were selected. The chronic nephropathy model was induced by intravenous injection of cationic bovine serum albumin (C-BSA) through the ear edge every day in 32 rats. The other 6 rats were not injected with C-BSA as control group. The hardness of renal cortex was measured by VTQ at 0 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks, and then the rabbits were dissected. To assess the pathological changes of the kidney, The correlation between VTQ parameters and pathological parameters was analyzed. Results the VTQ values of the renal cortex of rabbits were 1.68 卤0.25 卤0.31 卤0.31 m / s / h and 2.19 卤0.31 卤0.31 卤0.31 ms-1 / s / d respectively, respectively. There was significant difference between the VTQ values of the renal cortex at 0 weeks and 4 weeks and 4 weeks and 4 weeks, respectively. There was a significant difference between the VTQ values of 8 weeks and 4 weeks and 6 weeks of renal cortex, respectively, and 4 weeks and 6 weeks of renal cortex, respectively, and the differences were statistically significant (P 0.05%, P 0.05%, P 0.05%, P 0.05%, P 0.05%, P 0.05%, P < 0.05), and the values of VTQ at 8 weeks and 6 weeks, respectively, were significantly different from those at 4 weeks and 6 weeks, respectively. The glomerulosclerosis index (GSI) was 0.81 卤0.40U 1.43 卤0.46V 2.15 卤0.46, and the area of collagen fibers in the renal interstitium was 26.28 卤11.062.37 卤10.09, respectively, which was significantly higher than that at 0 week and 2 weeks, respectively, and the glomerulosclerosis index was 0.81 卤0.401.43 卤0.46U 2.15 卤0.46, respectively, and the area of collagen fibers in the renal interstitium was 26.28 卤11.062.37 卤10.09, which was significantly higher than that at 0 week and 2 weeks. The renal cortex VTQ was positively correlated with glomerulosclerosis index and interstitial collagen fiber area. Conclusion VTQ can detect the hardness of renal cortex during the development of chronic nephropathy in rabbits. Positive correlation with renal fibrosis suggests that VTQ may be an important method for evaluating renal fibrosis.
【作者单位】：上海交通大学附属第一人民医院超声科;上海市嘉定区中心医院超声科;
【基金】：国家自然科学基金项目(81171352) 上海市卫生局科研课题项目(20134287)
【分类号】：R-33;R445.1

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