猫小脑皮质结构年龄相关变化的研究

发布时间：2018-02-09 06:39

本文关键词： 猫小脑皮质衰老 Nissl染色免疫组织化学胶质纤维酸性蛋白神经丝蛋白 S100蛋白浦肯野细胞超微结构　出处：《安徽师范大学》2006年硕士论文　论文类型：学位论文

【摘要】： 小脑是调节躯体运动的高级中枢,主要接受与运动有关的信息,包括本体感觉信息和部分外周感觉信息,这些与运动有关的信息汇聚到小脑浦肯野细胞(PC),而PC的活动又由其轴突通过小脑核作用到脊髓、脑干及大脑皮层。衰老造成小脑皮质的形态结构、细胞构筑及神经递质等方面的改变,可能导致老年人和动物小脑功能衰退。猫是一类比较高级的哺乳动物,衰老过程中,猫中枢神经系统结构和功能改变已有广泛研究,但其小脑皮质年龄相关的形态学变化未见报道。本文对青年猫和老年猫小脑皮质结构的差异进行比较研究,以期为探讨老年小脑功能衰退的神经机制积累形态学资料。 1.以青年猫(1-3龄)和老年猫(12-13龄)小脑皮质为研究对像,运用Nissl染色显示小脑皮质神经元及皮质分层结构,免疫组织化学ABC法显示星形胶质细胞特异性标志物胶质纤维酸性蛋白免疫阳性(Glial fibrillary acidic protein immunoreactive,GFAP-IR)星形胶质细胞和神经丝蛋白免疫阳性(Neurofilament immunoreactive,NF-IR)神经元。与青年猫相比,老年猫小脑皮质总厚度及分子层厚度显著下降,颗粒层厚度明显增加;各层神经元密度明显降低;PC胞体收缩,树突分枝大量丢失;颗粒层中GFAP-IR细胞密度显著增大,阳性反应增强。推测衰老过程中小脑皮质神经元丢失,PC树突野萎缩,可能是导致小脑皮质接受和整合信息功能退化的重要原因之一;老年个体小脑皮质中星形胶质细胞活动增强对皮质神经元可能起保护作用。 2.运用电镜技术观察青年猫和老年猫小脑PC的超微结构。结果显
[Abstract]:The cerebellum is the advanced center that regulates the body movement. It mainly receives the information related to movement, including proprioceptive information and some peripheral sensory information. This motion-related information is gathered into the Purkinje cells of the cerebellum, and the activity of PC is activated by its axons through the cerebellar nucleus to the spinal cord, the brainstem and the cerebral cortex. Aging results in the morphology of the cerebellar cortex. Changes in cellular architecture and neurotransmitters may lead to the decline of cerebellar function in the elderly and animals. However, the age-related morphological changes of cerebellar cortex were not reported. The differences of cerebellar cortex structure between young cats and old cats were studied in order to accumulate morphological data for exploring the neuromechanism of cerebellar function decline in elderly cats. 1. The cerebellar cortex of young cats (1-3 years old) and old cats (12-13 years old) were studied. The cerebellar cortical neurons and cortical stratified structure were demonstrated by Nissl staining. Immunohistochemical ABC method showed astrocytes and neurofilaments immunoreactive NF-IRN neurons in astrocytes, glial fibrillary acidic protein immunoreactive protein, glial fibrillary acidic protein (GFAP-IRI) and neurofilaments immunoreactive (NF-IRR) neurons in young cats. The total thickness of cerebellar cortex and the thickness of molecular layer decreased significantly, the thickness of granular layer increased significantly, the density of neurons in each layer decreased the contraction of PC cell body and the loss of dendritic branches, and the density of GFAP-IR cells in granular layer increased significantly. It is speculated that the loss of cerebellar cortical neurons and atrophy of PC dendritic field during senescence may be one of the important reasons for the degeneration of cerebellar cortex reception and integration information function. Enhanced astrocyte activity in the cerebellar cortex may protect cortical neurons in the elderly. 2. The ultrastructure of cerebellar PC in young and old cats was observed by electron microscope.
【学位授予单位】：安徽师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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