兔氯胺酮—氟哌啶复合麻醉的实验研究

发布时间：2018-02-15 10:18

  本文关键词： 兔 氯胺酮-氟哌啶 氯胺酮 氯胺酮-芬太尼 复合麻醉　出处：《南京农业大学》2005年硕士论文　论文类型：学位论文

【摘要】：实验动物科学是生命科学研究的基础和重要的支撑条件。人类各种疾病发生发展十分复杂，要想深入探讨疾病的发病及防治机制，被称为“活的精密仪器”的实验动物，有着不可替代的作用。近年来，新西兰兔由于个体的种种特性及优势，而被我院广泛地应用于动物外科实验。但是，兔的麻醉问题常常困扰着我们，一方面是可供选择的麻醉药物较少(如常用的硫喷妥钠、戊巴比妥钠现已在市场上难以购到；氨基甲酸乙酯由于其自身的毒性，，现已逐渐被淘汰)，特别是我院所用的麻醉药品均是临床外科中人用的麻醉药，缺乏专门用于兔的麻醉药品；另一方面有关麻醉药对兔生理机能的影响现在还知之甚少。因此，本实验的目的是研究—种理想的兔复合麻醉方法，并探讨复合麻醉对兔麻醉效果及其生理机能的影响。 实验Ⅰ 兔氯胺酮与氟哌啶复合麻醉的实验研究 本实验目的是观察应用不同细合的氯胺酮与氟哌啶对兔复合麻醉效果。方法：48只健康新西兰兔随机分为6组，即Ⅰ(50mg氯+2.5mg氟)、Ⅱ(50mg氯+5mg氟)、Ⅲ(75mg氯+2.5mg氟)、Ⅳ(75mg氯+5mg氟)、Ⅴ(100mg氯+2.5mg氟)和Ⅵ(100mg氯+5mg氟)组，分别肌肉注射，通过观察麻醉出现时间(以动物自然倒下、睫毛反射及角膜反射减弱为有效指标)、麻醉维持时间(以麻醉过程平稳、胸腹式呼吸、肌肉完全松弛及钳夹趾间组织偶尔可引起趾蹼反射为有效指标)、麻醉苏醒时间(以头抬起、肢体躁动及有痛感为有效指标)进行麻醉效果的评价(优：术中无肢动行为；良：术中曾有影响手术操作的肢动活动；差：术中肢体乱动，手术无法进行；死亡率：从手术开始至术后24h内有无死亡为有效指标)。实验结果显示，Ⅰ、Ⅱ细麻醉效果差，麻醉维持时间短，分别为37±3min和42±3min。Ⅲ细麻醉效果良，麻醉维持时间为40±3min。Ⅳ、Ⅴ、Ⅵ细麻醉效果优；但Ⅴ组麻醉维持时间较短(42±5min)，Ⅵ组虽麻醉维持时间长(75±5min)，但却有死亡；Ⅳ组(75mg氯+5mg氟)·kg~(-1)术中肌肉松弛，无肢动行为，麻醉维持时间在45±5min，无死亡发生。结论：Ⅳ组(75mg氯胺酮·kg~(-1)+5mg氟哌定·kg~(-1)的麻醉效果最好，可满足45min左右的手术实验。
[Abstract]:Laboratory animal science is the foundation and important supporting condition of life science research. The occurrence and development of human diseases is very complicated. To study the pathogenesis and prevention mechanism of diseases, it is called "living precision instrument" experimental animals. In recent years, New Zealand rabbits have been widely used in animal surgery experiments because of their individual characteristics and advantages. On the one hand, there are fewer narcotic drugs available (such as the commonly used thiopental sodium, pentobarbital sodium, which is now difficult to buy on the market; ethyl carbamate) because of its own toxicity, The narcotic drugs used in our hospital have been gradually eliminated, especially those used in clinical surgery for human use and lack of narcotic drugs specifically used in rabbits. On the other hand, little is known about the effects of anesthetics on the physiological functions of rabbits. The purpose of this experiment was to study the ideal method of compound anesthesia in rabbits and to explore the effect of compound anesthesia on the anesthetic effect and physiological function of rabbits. Experimental study on compound Anesthesia of Ketamine and Haloperidol in Rabbits. The purpose of this experiment was to observe the effect of different fine combination ketamine and haloperidol on rabbit compound anesthesia. Methods Twenty eight healthy New Zealand rabbits were randomly divided into 6 groups. Groups 鈪

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