甘氨酸对小鼠脂质代谢的影响及对肝脂肪变性的抑制作用

发布时间：2018-02-21 00:36

本文关键词： 甘氨酸脂质代谢高脂血症肝脂肪变　出处：《暨南大学》2005年硕士论文　论文类型：学位论文

【摘要】：目的:观察甘氨酸(glycine,GLY)对小鼠脂质代谢的影响及其抑制高脂血症小鼠脂肪肝形成的作用及机制。方法:用连续喂养高脂饲料的方法建立高脂血症小鼠模型,小鼠随机分为对照组(control group),正常饮食的5%GLY组、10%GLY组、20%GLY组,高脂饮食组(high-fat diet group,HF)、HF+5%GLY组、HF+10%GLY组、HF+20%GLY组,观察不同剂量GLY(5%、10%、20%)对小鼠体脂含量和血脂代谢(TC、TG、HDLC、LDL)的影响,并测定血清中瘦素(Leptin)的含量。并用同样的方法建立高脂血症小鼠模型,观察20%GLY对高脂血症小鼠脂肪肝形成的影响,同时测定肝指数、肝组织中丙二醛(malondialdehyde,MDA)的含量、肝组织总蛋白含量以及超氧化物歧化酶(superoxide dismutase,SOD)的活性,并留取肝组织做病理形态学的观察。结果:(1) 体脂含量:雌雄小鼠正常饮食20%GLY组体脂含量明显低于对照组,且高脂饮食20%GLY组体脂含量明显低于HF组,雌性小鼠HF+10%GLY组体脂含量也明显低于HF组;(2) 血脂:高脂饮食GLY各组小鼠血清TC和LDL水平明显低于HF组;(3) 血清leptin:雌雄小鼠HF组血清中leptin含量明显高于对照组,雄性小鼠5%和10%GLY组血清leptin含量明显高于对照组,雌雄小鼠HF+5%GLY组血清leptin含量明显高于HF组;(4) SOD、MDA、和总蛋白含量:高脂饮食的20%GLY组肝组织中SOD的活性明显高于HF组,MDA的含量明显低于HF组,肝组织总蛋白含量明显高于HF组;(5) 肝脂肪变性:肝组织病理形态学显示,高脂饮食的各剂量GLY组肝脂肪变性明显轻于HF组。结论:(1) 一定剂量的GLY能够明显降低体脂含量,可能和GLY直接或间接促进leptin分泌有关。(2) 不同剂量的GLY对正常饮食小鼠的血脂影响不大,对高脂饮食的小鼠则有明显降低其血中TC和LDL含量的作用。(3) 一定剂量的GLY对高脂血症肝脂肪变形成有抑制作用,能够明显降低肝指数,降低肝组织中过氧化物MDA的含量,升高肝组织总蛋白含量,增强肝中抗氧化酶类SOD的活性,抑制氧化应激,保护肝组织。
[Abstract]:Aim: to observe the effects of glycine glycine glycine (GLY) on lipid metabolism and the mechanism of inhibiting fatty liver formation in hyperlipidemic mice. Methods: the hyperlipidemia mice model was established by continuous feeding high fat diet. Mice were randomly divided into control group (control group), GLY group (10 GLY group) and GLY group (20 GLY group), high-fat diet group (HF5GLY) group (HF10GY group), HF20GLY group (HF20GLY group), high fat diet group (HF5GLY group), high fat diet group (HF10GLY group) and HF20GLY group (HF20GLY group). To observe the effects of different doses of GLY5 and Rhizoma Rhizoma Ritchii on the content of body fat and the metabolism of blood lipid in mice, and to determine the content of leptin (Leptin) in serum, and to establish the model of hyperlipidemia mice with the same method, to observe the effect of 20 GLY on the formation of fatty liver in hyperlipidemic mice, and to observe the effect of GLY on the formation of fatty liver in hyperlipidemic mice, and to observe the effect of GLY on fatty liver formation in hyperlipidemic mice. At the same time, the liver index, the content of malondialdehyde (malondialdehyde), the content of total protein and the activity of superoxide dismutase (SOD) in liver tissue were measured, and the histopathologic changes of liver tissue were observed. Results: the body fat content of female and male mice in normal diet 20GLY group was significantly lower than that of control group, and the body fat content of high fat diet 20g GLY group was significantly lower than that of HF group. The serum TC and LDL levels of female mice in HF10GLY group were significantly lower than that of HF group (P < 0.05). The serum TC and LDL levels in GLY group were significantly lower than those in HF group (P < 0.05). The content of leptin in HF group was significantly higher than that in control group. The serum leptin levels of male mice in 5% and 10 GLY groups were significantly higher than those in control group. The contents of serum leptin and total protein in HF5GLY group were significantly higher than those in HF group. The activity of SOD in liver tissue of 20g Gly group with high fat diet was significantly higher than that of HF group, which was significantly lower than that of HF group. The content of total protein in liver tissue was significantly higher than that in HF group (P < 0.05). The histopathology of liver tissue showed that the liver steatosis of GLY group with high fat diet was significantly lighter than that of HF group. Conclusion: GLY at a certain dose can significantly decrease body fat content, which may be related to the direct or indirect promotion of leptin secretion by GLY.) different doses of GLY have little effect on blood lipid of normal diet mice. On the other hand, the mice with high fat diet had the effect of decreasing the contents of TC and LDL in their blood. The effect of a certain dose of GLY on the formation of hepatic fat in hyperlipidemic mice could significantly decrease the liver index and the content of peroxide MDA in liver tissue. The content of total protein in liver tissue was increased, the activity of antioxidant enzymes SOD in liver was enhanced, oxidative stress was inhibited and liver tissue was protected.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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