SARS病毒特异性抗原抗体基因和N基因的研究

发布时间：2018-02-24 22:33

本文关键词： SARS病毒噬菌体展示单链抗体酶联免疫吸附检测细胞凋亡 S蛋白 N蛋白辅酶Q-细胞色素C还原酶Fe-S蛋白　出处：《武汉大学》2005年博士论文　论文类型：学位论文

【摘要】：SARS于2002年11月16日首先在中国广东省佛山出现,然后迅速在全世界30多个国家或地区相继爆发流行。它是由一种从未在人类身上发现的冠状病毒(命名为“SARS病毒”)引起的、并具有高发病率和致死率的新传染性疾病。由于缺乏统一的检测、诊断标准和手段以及有效的疫苗和治疗药物,在短短10个月内,导致了8000多人染病,800多人死亡。尽管在2003年末,SARS在全世界范围内最终得到了控制,但是,在新加坡、中国台湾和大陆仍有一些零星的病例相继被报道,说明SARS有可能再次出现。因此,不管是在SARS爆发期间,或者为了应对SARS的可能再次出现,发展可靠的早期检测、诊断试剂以及有效或高效的抗SARS病毒疫苗和治疗药物一直都是各国科学家所共同关心的热点问题。目前,SARS冠状病毒检测和诊断技术的研究和开发主要包括几个方面(Poon et al.,2003):基于病毒抗原-抗体反应的免疫测定法;针对病毒RNA的核酸检测技术、通过细胞培养和病毒分离的病毒培养法以及生物芯片技术。用免疫学方法进行感染性疾病的诊断,一般包括两个方面:1) 检测微生物的特异性抗原;2) 检测微生物的特异性抗体。不管怎样,目前正在研究和开发的ELISA(enzyme-linked immunosorbent assay)检测试剂盒无论是作为一种辅助鉴定SARS的临床检测手段,还是确诊手段都是非常有用的,可以认为是当前鉴定SARS感染的最有效的免疫学检测手段。因此,为了发展针对SARS-CoV的免疫学早期诊断试剂,我
[Abstract]:In November 16th 2002, SARS first appeared in Foshan, Guangdong Province, China, and then spread rapidly in more than 30 countries and regions around the world. It was caused by a coronavirus (named "SARS virus"), which has never been found in humans. And new infectious diseases with high morbidity and mortality. Due to the lack of uniform testing, diagnostic criteria and means, and effective vaccines and treatment drugs, within a short period of 10 months, More than 8, 000 people were infected and more than 800 died. Although SARS was finally brought under control worldwide in end of 2003, sporadic cases were reported in Singapore, Taiwan and mainland China. Therefore, whether during the outbreak of SARS, or in response to the possible re-emergence of SARS, the development of reliable early detection, Diagnostic reagents, effective or effective antiviral vaccines and therapeutic drugs have been a hot issue of common concern by scientists all over the world. At present, the research and development of detection and diagnosis technology for SARS coronavirus mainly include several aspects: immunoassay based on virus antigen-antibody reaction, nucleic acid detection technology for virus RNA, Through cell culture, virus culture for virus isolation and biochip technology, immunological methods are used for the diagnosis of infectious diseases, In general, there are two aspects: 1) Detection of specific microorganism antigens 2) Detection of specific antibodies of microbes. Anyway, the ELISA(enzyme-linked immunosorbent assay kit currently under study and development, whether as a clinical means to assist in the identification of SARS, It can be considered as the most effective immunological method for the identification of SARS infection. Therefore, in order to develop the immunological early diagnostic reagent for SARS-CoV, I
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R373

【共引文献】