宫内感染致脑损伤对仔鼠认知发育和海马神经发生的影响

发布时间：2018-02-26 13:14

本文关键词： 宫内感染脑损伤神经细胞凋亡认知发育神经发生　出处：《中国病理生理杂志》2014年11期 　论文类型：期刊论文

【摘要】：目的:探讨宫内感染致仔鼠脑损伤后对认知发育和海马神经发生的影响。方法:15只SpragueDawley孕鼠(孕15 d)按随机数字表法分为造模组(8只)和对照组(7只)。繁殖后的2组雄性仔鼠按随机数字表法分为宫内感染组(35只)和对照组(35只),进行大脑解剖学观察、神经细胞凋亡检测、Morris水迷宫实验、神经细胞增殖及存活分析。结果:(1)宫内感染组仔鼠大脑较对照组萎缩,TUNEL阳性细胞数和caspase-3阳性细胞数均较对照组显著增加(P0.05)。(2)宫内感染组仔鼠近期记忆及远期记忆保持能力均较对照组降低,潜伏期显著延长(P0.05)。(3)细胞增殖分析显示宫内感染组3、7和14日龄仔鼠Brd U阳性细胞数较对照组显著增加(P0.05);细胞存活分析显示,28日龄的2组仔鼠Brd U阳性细胞数无显著差异(P0.05)。结论:宫内感染可致仔鼠脑损伤,海马神经细胞凋亡可能与认知发育障碍密切相关。宫内感染诱导的神经发生可能与脑损伤后神经自身修复有关。
[Abstract]:Objective: to investigate the effects of intrauterine infection on cognitive development and hippocampal neurogenesis in postnatal rats. Methods: 15 SpragueDawley pregnant rats (15 days pregnant) were randomly divided into model group (n = 8) and control group (n = 7). Male rats in group A were randomly divided into intrauterine infection group (n = 35) and control group (n = 35). Morris water maze test for neuronal apoptosis, Results compared with the control group, the number of Tunel positive cells and the number of caspase-3 positive cells in the brain of the rats in the intrauterine infection group increased significantly compared with the control group. The holding capacity was lower than that in the control group. The results of cell proliferation analysis showed that the number of Brd U positive cells in intrauterine infection group was significantly higher than that in control group, and cell survival analysis showed that there were no significant Brd U positive cells in 2 groups at 28 days old. Conclusion: intrauterine infection can cause brain injury in newborn. The apoptosis of hippocampal nerve cells may be closely related to cognitive dysplasia, and the induction of intrauterine infection may be related to nerve self-repair after brain injury.
【作者单位】：浙江大学医学院附属儿童医院神经内科;浙江大学医学院附属邵逸夫医院ICU;
【基金】：国家自然科学基金资助项目(No.81201511) 浙江省医药卫生科技计划(No.2010KYB064) 浙江省教育厅科研项目(No.Y201225805) 浙江省人口计生委科研项目(No.JSW2012-A011)
【分类号】：R363

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