幽门螺杆菌适应性蛋白的初步研究

发布时间：2018-02-27 06:25

本文关键词： 幽门螺杆菌双向凝胶电泳基因缺失突变　出处：《山东大学》2005年硕士论文　论文类型：学位论文

【摘要】：幽门螺杆菌(Helicobacter pylori,H. pylori)作为唯一能在人体胃内酸性环境中定植并长期生存的致病菌,其生长在体内必然会受到各种不利条件的影响,H. pylori通过启动一系列的适应性调节机制来抵抗这些胁迫因素以达到保种生存并导致疾病的目的。形态学变化是H. pylori的适应性调控机制之一,在不同的胁迫条件下H. pylori可发生不同的形态学变化,蛋白质是生命活动的最终执行者,无论何种表征蛋白质在其中将起重要作用,比较蛋白质组学是研究病原菌在各种胁迫因素下蛋白质表达差异的理想工具,现今较普遍使用的技术体系包括双向凝胶电泳,质谱分析及生物信息学分析。胆汁是机体重要的天然抗病原菌物质,研究H. pylori在胆汁中的存活能力,对于揭示H. pylori感染与十二指肠溃疡的发生规律有重要意义,为此我们研究了H. pylori在胆汁胁迫下的蛋白表达状态。丝状体是H. pylori除螺杆状和球形体以外的一种成活形式,对丝状体蛋白表达谱的研究,有助于揭示其发生规律。我们曾发现鞭毛丝帽蛋白与H. pylori结构性群体结构形成密切相关,然而,对生物质谱筛选的候选蛋白尚需作进一步的功能验证,为此,我们构建了H. pylori结构性群体形成相关蛋白鞭毛丝帽蛋白基因缺失突变株,为进一步验证此蛋白的功能及阐明H. pylori结构性群体的形成机制奠定工作基础。主要研究内容及研究结果如下: 一、胆汁对幽门螺杆菌的抑制及其蛋白质表达谱的初步研究胆汁胁迫是人体内抑制H. pylori生长的不利条件之一,许多研究也表明胆汁能够抑制H. pylori的生长,但H. pylori仍能够在胆汁丰富的十二指肠球部存在,而对其他肝胆系统的感染较少见,因此我们推测胆汁的酸化影响其对H. pylori的抑制活性。为了研究H. pylori对胆汁胁迫的适应性调
[Abstract]:Helicobacter pylori Helicobacter pylori H.pylori is the only bacterium that can colonize and survive in an acidic environment in the stomach of a human body. Its growth in vivo is bound to be affected by various adverse conditions. H. pylori protects against these stress factors by initiating a series of adaptive regulatory mechanisms to preserve the survival of species and lead to disease. Morphological changes are H. pylori. One of the adaptive regulatory mechanisms, Under different stress conditions, the morphological changes of H. pylori are different. Protein is the final executor of life activity, no matter what characterization protein will play an important role in it. Comparative proteomics is an ideal tool for studying the differences in protein expression of pathogens under various stress factors. Bile is an important natural antipathogenic substance in the body. It is important to study the survival ability of H. pylori in bile for revealing the regularity of H. pylori infection and duodenal ulcer. In this paper, we studied the protein expression of H. pylori under bile stress. The filamentous body is a survival form of H. pylori except the screw and spheroids, and the protein expression profile of the filamentous body is studied. We have found that flagellum cap protein is closely related to the formation of structural population structure of H. pylori. However, the candidate proteins screened by mass spectrometry need further functional verification. We constructed a mutant with deletion of flagellin gene associated with the structural colony formation of H. pylori. In order to further verify the function of the protein and elucidate the formation mechanism of the structural population of H. pylori, the main contents and results are as follows:. A preliminary study on the inhibition of bile on Helicobacter pylori and its protein expression profile. Bile stress is one of the unfavorable conditions for inhibiting the growth of H. pylori in human body. Many studies have also shown that bile can inhibit the growth of H. pylori, but H. pylori can still exist in the duodenal bulb where bile is abundant, but infection of other hepatobiliary systems is rare. Therefore, we speculate that the acidification of bile affects the inhibitory activity of H. pylori. In order to study the adaptability of H. pylori to bile stress
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R378

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