兴奋缰核对老年大鼠颏舌肌和膈肌肌电的影响

发布时间：2018-02-28 20:50

本文关键词： 缰核中缝背核阻塞性睡眠呼吸暂停综合症 5-羟色胺 γ-氨基丁酸　出处：《吉林大学》2006年硕士论文　论文类型：学位论文

【摘要】： 本实验目的在于探讨电刺激及化学刺激缰核(Hb)对老年大鼠呼吸,颏舌肌、膈肌肌电的影响,以进一步探明上述核团在睡眠呼吸暂停综合症(SAS)的发病中的作用。采用电刺激、电损毁和微量注射的方法。结果:1.电刺激LHb,可迅速产生类似阻塞性睡眠呼吸暂停综合症(OSAS)的呼吸状态,刺激停止后逐渐恢复至电刺激前呼吸状态。呼吸频率降低(38%),颏舌肌和膈肌肌电改变表现双向反应,先出现瞬间的降低而后反射性升高,并且以升高明显。颏舌肌肌电积分值下降(22%),然后快速升高至(104%)。膈肌肌电积分值降低(11%),然后迅速反射性升高(23%)。微小电刺激MHb后,呼吸频率增加(12%)。刺激LHb和MHb对动物的呼吸产生不同的效应,低强度刺激MHb引起动物呼吸加快,而刺激LHb动物呼吸出现类似OSAS呼吸状态,呼吸频率变慢,这可能是不同类型的神经元被兴奋所致。颏舌肌和膈肌肌电出现先降低而后增加的双向反应,尤其以增强效应为主,可能是出现类似OSAS状态后机体自身的反馈调节所致,其中机制有待进一步的研究。2.电损毁大鼠Hb后呼吸频率升高(19%),颏舌肌肌电积分值降低(46%),膈肌肌电积分值下降(19%),电损毁缰核后得到的结果不是很明显,但是依然有统计意义。3. Hb内微量注5-羟色胺(5-HT),呼吸频率降低(31%),颏舌肌和膈肌肌电改变表现双向反应,先出现瞬间的降低而后反射性升高,并且以升高明显。颏舌肌肌电变化积分值下降(21%),然后快速升高(100%)。膈肌肌电积分值降低(14%)。然后迅速反射性升高(24%)。4.Hb内微量注射γ-氨基丁酸(GABA),呼吸频率增加(26%),颏舌肌肌电积分值下降(33%),膈肌肌电积分值下降(8%)。实验结果证明Hb与中缝背核之间有密切的往返纤维联系,初步确定5-HT及GABA是Hb影响中缝背核活动的介导物质之一,
[Abstract]:The purpose of this study was to investigate the effects of electrical and chemical stimulation of habenular nucleus (HB) on respiration, genioglossus and diaphragmatic myoelectric activity in aged rats, in order to further explore the role of these nuclei in the pathogenesis of sleep apnea syndrome (SAS). Electrical damage and microinjection. Results: 1. Electrical stimulation of LHb can rapidly produce respiratory states similar to obstructive sleep apnea syndrome (OSA). After the stimulation stopped, it gradually returned to the breathing state before electrical stimulation. The respiratory rate was reduced by 38%. The electromyography of genioglossus and diaphragm showed a bidirectional response, with a transient decrease and then a reflex increase. And the increase was obvious. The score of genioglossus decreased 22 times, and then rapidly increased to 104. The integral value of diaphragm decreased 11%, and then the rapid reflex increased 23%. After micro electrical stimulation of MHb, The stimulation of LHb and MHb had different effects on the respiration of animals. Low intensity stimulation of MHb increased the rate of respiration in animals, while stimulating LHb resulted in a similar OSAS respiratory state, and the respiratory rate slowed down. This may be due to the excitation of different types of neurons. The bidirectional response of the genioglossus and the diaphragm muscle to decrease first and then increase, especially the enhancement effect, may be due to the feedback regulation of the body itself after the appearance of a similar OSAS state. Among them, the mechanism needs further study .2.Respiratory frequency increased after HB was damaged by electrolysis. The electromyography score of genioglossus decreased by 46%, the integral value of diaphragmatic muscle decreased by 19%, and the results obtained after electric lesion of habenular nucleus were not very obvious. However, there is still statistical significance .3.The microinjection of 5-hydroxytryptamine into HB reduces the respiratory rate by 31%. The electromyography of genioglossus and diaphragm shows a two-way response, with a transient decrease and then an increase in reflex. The integral value of genioglossus electromyography decreased by 21%, and then increased by 100%. The diaphragmatic myoelectric integral value decreased 14%. Then the rapid reflex increased 24%. 4. Microinjection of GABA A into HB, the respiratory rate increased 26%, and the genioglossus muscle increased 26%, and the muscle of the genioglossus increased by 26%, and the muscle of the genioglossus increased by 26%, and the muscle of the genioglossus increased by 26%. The decrease of electrical integral value and the decrease of diaphragmatic myoelectric integral value show that there is a close fiber connection between HB and the dorsal raphe nucleus, and the results of the experiment show that there is a close connection between HB and the dorsal raphe nucleus. 5-HT and GABA were identified as one of the mediators of HB affecting the activity of dorsal raphe nucleus.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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