大鼠“肾阳虚证”下急性心肌梗死模型的建立与评价

发布时间：2018-03-01 13:29

本文关键词： 肾阳虚证急性心肌梗死血液流变学血流动力学　出处：《吉林大学》2007年硕士论文　论文类型：学位论文

【摘要】： 在目前的中药药理研究中,绝大多数动物实验还是采用西医病因病理复制动物模型,只强调“病”,而忽略了“证”,很难通过动物模型反映中医“辨证施治”的治疗原则。例如当前在研究治疗胸痹心痛的中药时,通常选用大鼠急性心肌梗死病理模型进行评价,忽视了中医的征候。而临床上的胸痹心痛可见多种中医的“证”,如“肾阳虚证”。但单纯西医的病理模型是否可以代替中医的“证”与“病”并存的征候模型,尚无前人报道。为确证“病”和“证”相统一的实验动物模型是否能科学的评价中药的药效特点,以便更好地指导中药的新药开发和研究,我们在大鼠皮下注射醋酸氢化可的松建立肾阳虚模型的基础上,结扎冠状动脉前降支24h建立了“肾阳虚证”下急性心肌梗死模型,观测形态学、血液流变学、血流动力学、心血管系统相关因子等指标,同时采用西药(硝酸甘油注射液)和中药(苦碟子注射液)进行药物干预,探讨单纯急性心肌梗死与“肾阳虚证”下急性心肌梗死的相关性。结果表明,心肌形态学、血液流变学、血流动力学、心血管系统相关因子等指标变化程度在“单纯”急性心肌梗死模型与“肾阳虚证”下急性心肌梗死模型之间没有明显差异,而且与西药和中药的干预作用结果亦相一致。提示在研究治疗胸痹心痛中药时,大鼠“单纯”急性心肌梗死可直接作为评价药效的模型,无须建立“肾阳虚证”与“急性心肌梗死”并存的实验动物模型。
[Abstract]:In the current pharmacological study of traditional Chinese medicine, the majority of animal experiments are still using Western medicine etiology and pathology to make animal models. It is difficult to reflect the treatment principle of TCM "differentiation and treatment" through animal models. For example, in the current research on traditional Chinese medicine for treating chest arthralgia and heart-ache, the pathological model of acute myocardial infarction in rats is usually selected for evaluation. The clinical symptoms of TCM were ignored, and many kinds of TCM "syndromes" could be seen in clinical chest arthralgia and heartache pain, such as "Kidney-Yang deficiency Syndrome". However, whether the pathological model of pure western medicine can replace the symptom model of TCM "syndrome" and "disease" has not been reported before. In order to confirm whether the unified animal model of "disease" and "syndrome" can scientifically evaluate the pharmacodynamic characteristics of traditional Chinese medicine, in order to better guide the development and research of new drugs of traditional Chinese medicine, On the basis of hypodermic injection of hydrocortisone acetate to establish the model of kidney yang deficiency, we established the acute myocardial infarction model under "kidney yang deficiency syndrome" after 24 h ligation of anterior descending branch of coronary artery, and observed morphology, hemorheology and hemodynamics. Cardiovascular system related factors and western medicine (nitroglycerin injection) and Chinese medicine (Kudiezi injection) were used to study the correlation between pure acute myocardial infarction and acute myocardial infarction under "deficiency of kidney-yang syndrome". The results showed that myocardial morphology, hemorheology, hemodynamics, There was no significant difference in the changes of cardiovascular system related factors between the "pure" acute myocardial infarction model and the "kidney-yang deficiency" acute myocardial infarction model. The results are consistent with those of western medicine and traditional Chinese medicine. It is suggested that "simple" acute myocardial infarction can be used as a model for evaluating drug efficacy in the study of traditional Chinese medicine for the treatment of chest arthralgia and heart-ache. There is no need to establish the experimental animal model of "Kidney Yang deficiency Syndrome" and "Acute Myocardial Infarction".
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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