大鼠臂旁核内神经元型一氧化氮合酶参与内脏伤害性刺激传导的研究

发布时间：2018-03-02 15:06

  本文选题：臂旁核　切入点：中央杏仁核　出处：《南京医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：1．观察内脏伤害性刺激后，大鼠臂旁核(PBN)内被激活神经元的亚核定位情况；2．探讨PBN内神经元型一氧化氮合酶(nNOS)是否参与对内脏伤害性刺激传导过程的调节；3．探讨臂旁核-中央杏仁核通路(PBN-CeA)是否参与对内脏伤害性刺激的上传过程，并阐明nNOS是否参与了对此传导通路的调节。 方法：1．给予大鼠2％福尔马林溶液灌胃后，用Fos蛋白免疫组织化学方法观察PBN内Fos阳性神经元在各亚核内的分布情况；2．用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)组织化学结合Fos蛋白免疫组织化学双重染色的方法，观察内脏伤害性刺激后，Fos阳性神经元和nNOS阳性神经元及纤维在PBN各亚核内的分布与共存情况；3．大鼠CeA内注射荧光金(FG)后，用FG、Fos免疫荧光结合NADPH-d组织化学三标染色的方法，观察内脏伤害性刺激后Fos、FG和nNOS在PBN内的分布及共存情况。 结果：1．同本实验室既往观察结果类似，，在正常大鼠PBN内具有NADPH-d的广泛分布：TypeⅠ型NADPH-d阳性神经元颜色深染、突起较长，主要分布在PBN的嘴侧部分；TypeⅡ型NADPH-d阳性神经元染色较淡，突起较短，主要沿PBN的背外侧界分布；PBN嘴侧可见大量的近似水平方向走行的纤维束穿过小脑上脚(scp)，臂旁外侧核外亚核(PBel)内可见密集点状分布的NADPH-d阳性纤维终末。2．内脏伤害性刺激后，Fos阳性神经元在PBN内的分布具有亚核特异性：PBel、K-F核和臂旁外侧核背亚核(PBdl)有大量分布；臂旁外侧核中央亚核有少量分布；其余各
[Abstract]:Objective: 1.After observing visceral nociceptive stimulation, Subnuclear localization of activated neurons in parabrachial nucleus of rats 2. To investigate the role of neuronal nitric oxide synthase (nNOS) in the conduction of visceral nociceptive stimulation in PBN. To explore the pathway of parabrachial nucleus to central amygdaloid nucleus (PBN-CeA). Whether or not to participate in the process of uploading visceral noxious stimuli, And whether nNOS is involved in the regulation of this transduction pathway. Methods 1. Rats were given 2% formalin solution. The distribution of Fos positive neurons in the subnuclei of PBN was observed by immunohistochemical method of Fos protein. The double staining method of NADPH-dH histochemistry combined with Fos protein was used to detect the distribution of Fos positive neurons in the subnuclei of PBN by using reduced nicotinamide adenine dinucleotide dehydrogenase (NADPH-d). To observe the distribution and coexistence of Fos positive neurons and nNOS positive neurons and fibers in the subnuclei of PBN after visceral nociceptive stimulation 3.After the rats were injected with FG into CeA, the FGS-Fos immunofluorescence and NADPH-d histochemical staining were used. After visceral nociceptive stimulation, the distribution and coexistence of Fosson-FG and nNOS in PBN were observed. Results (1) similar to the previous observations in our laboratory, there was a widespread distribution of NADPH-d in PBN of normal rats. The positive neurons of NADPH-d type 1 were dark stained and had long protrusions. The staining of type 鈪

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