高迁移率族蛋白B1对体外培养原代神经干细胞增殖能力的影响

发布时间：2018-03-03 15:41

本文选题：神经干细胞　切入点：增殖　出处：《生理学报》2014年04期 　论文类型：期刊论文

【摘要】：本研究采用CCK-8增殖实验和测量神经球直径的方法探讨不同浓度高迁移率族蛋白B1(high-mobility group box 1,HMGB1)对体外培养原代大鼠海马神经干细胞增殖能力的影响,同时通过使用c-Jun氨基端激酶(c-Jun N-terminal kinase,JNK)高选择性抑制剂SP600125探讨其作用机制。结果显示,1和10 ng/mL HMGB1分别培养48 h和72 h后,神经干细胞的CCK-8吸光度值和神经球直径与对照组比较有明显增加(P0.05),而其余浓度HMGB1培养基(0.01、0.1、100 ng/mL)与对照组比较无明显差别(P0.05)。当HMGB1浓度为10 ng/mL时,在神经干细胞增殖的同时p-JNK水平明显升高(P0.01);而10μmol/L SP600125明显抑制HMGB1促进神经干细胞增殖的作用,降低p-JNK水平(P0.01)。结果表明,低浓度(1~10 ng/mL)HMGB1能够促进神经干细胞增殖,其机制可能是通过促进JNK的磷酸化发挥促增殖作用的。
[Abstract]:The aim of this study was to investigate the effects of high-mobility group group box 1HMGB1 at different concentrations on the proliferation of primary rat hippocampal neural stem cells (NSCs) cultured in vitro by means of CCK-8 proliferation test and measurement of the diameter of neurospheres. The mechanism was also investigated by using c-Jun N-terminal kinase (SP600125), a highly selective inhibitor of c-Jun N-terminal kinase. The results showed that 1 and 10 ng/mL HMGB1 were cultured for 48 h and 72 h, respectively. Compared with the control group, the absorbance of CCK-8 and the diameter of the nerve bulb of the neural stem cells increased significantly (P 0.05), while the other concentration of HMGB1 medium was 0.01g / mL. There was no significant difference between the control group and the control group (P 0.05). When the concentration of HMGB1 was 10 ng/mL, there was no significant difference between the two groups. At the same time of proliferation of neural stem cells, p-JNK level was significantly increased, while 10 渭 mol/L SP600125 significantly inhibited the effect of HMGB1 on the proliferation of neural stem cells, and decreased the level of p-JNK. The results showed that the low concentration of p-JNK 10 ng/mL)HMGB1 could promote the proliferation of neural stem cells. Its mechanism may play a proliferative role by promoting phosphorylation of JNK.
【作者单位】：山西医科大学第二医院神经内科;重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室;山西医科大学基础医学院;山西医学科学院山西大医院骨科;
【基金】：supported by the National Natural Science Foundation of China(No.30470606) the Traditional Chinese Medicine Research Program of Chongqing Municipal Health Bureau,China(No.2003-B-16)
【分类号】：R363

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