2型糖尿病心血瘀阻证病证结合大鼠模型的研制

发布时间：2018-03-04 07:11

本文选题：糖尿病　切入点：心血瘀阻　出处：《湖南中医药大学》2006年硕士论文　论文类型：学位论文

【摘要】： 目的:建立2型糖尿病心血瘀阻证的病证结合的实验动物模型,使糖尿病并发症的中医证型动物模型规范化、标准化,为临床糖尿病心血管并发症的有效治疗和中药新药开发创造良好条件。方法:将46只清洁级wistar大鼠常规饲养一周后,随机抽取10只为正常对照组,其余36只为造模组。造模组喂以高脂饲料4周后,再随机抽取12只为心血瘀阻证造模组(心血瘀阻证模型组),其余24只注射少剂量的链脲佐菌素行糖尿病造模,成模大鼠按血糖、体重分为两组,随机抽取一组为糖尿病模型组(糖尿病组),剩下一组利用强的松龙和肾上腺素行血瘀证造模,从而诱导出糖尿“病”心血瘀阻“证”病证结合的大鼠模型(糖尿病心血瘀阻证组)。结果: (1)一般状况:糖尿病心血瘀阻证组(糖尿病心血瘀证组)大鼠造模后出现精神萎糜,倦怠懒动,消瘦、大便量多、稀,小便稍多,舌上可见瘀点、甚可见瘀斑等症状为造模成功。 (2)糖尿病心血瘀证组与其它三组比较,血糖均有增高,差异具有显著性(p＜0.01或p＜0.05)。 (3)糖尿病心血瘀证组的心脏湿重与体重的比值和心电图结果与其他三组比较,差异具有显著性(p＜0.01或p＜0.05)。 (4)糖尿病心血瘀证组血液流变学多项主要指标值较其它三组比较,均有升高,差异具有显著性(p＜0.01或p＜0.05)。 (5)糖尿病心血瘀证组的球结膜管袢形态积分和流态积分与其它三组比较,差异具有显著性(p＜0.01或p＜0.05);而周态积分无异常(p＞0.05)。 (6)糖尿病心血瘀证组与其它三组比较,总胆固醇、甘油三脂有明显升高、高密度脂蛋白有明显降低,差异具有显著性(p＜0.01或p＜0.05)。 (7)光镜下,糖尿病心血瘀证组心肌细胞有炎症细胞浸润,细胞坏死和肌纤维断裂。 (8)光镜下,糖尿病心血瘀证组的主动脉有动脉斑块。结论:1.应用传统的糖尿病造模方法与糖尿病并发症病证特点和糖尿病发病的病因病理相结合而研制2型糖尿病心血瘀阻证的病证结合的动物模型是切实可行的。 2.在糖尿病模型的基础上再造血瘀证模型,从而诱导出糖尿病心血瘀证模型的造模方法,创立出糖尿“病”和心血瘀“证”相结合的病证结合的动物模型,此方法可推广。
[Abstract]:Objective: to establish an experimental animal model of the combination of disease and syndrome of type 2 diabetes mellitus with heart blood stasis syndrome, and to standardize and standardize the animal model of TCM syndrome of diabetic complications. To provide a good condition for the effective treatment of cardiovascular complications of diabetes mellitus and the development of new drugs of traditional Chinese medicine. Methods: after a week of routine feeding of 46 clean grade wistar rats, 10 rats were randomly selected as normal control group and the remaining 36 rats as model group. The model group was fed with high fat diet for 4 weeks. Twelve rats were randomly selected as heart blood stasis syndrome model group (heart blood stasis syndrome model group), the remaining 24 rats were injected with low dose streptozotocin to make diabetes model. The model rats were divided into two groups according to blood sugar and body weight. One group was randomly selected as diabetic model group (diabetes group), and the remaining group was treated with prednisolone and epinephrine to make model with blood stasis syndrome. Thus, the rat model of diabetes "disease" and "heart blood stasis" syndrome combined with disease and syndromes was induced. Results:. (1) General condition: the rats of diabetic heart blood stasis syndrome group (diabetes heart blood stasis syndrome group) appeared mental wilting, languid and lazy, wasting, defecation amount, dilute, urination a little more, blood stasis on the tongue, It can be seen that ecchymosis and other symptoms for successful modeling. (2) compared with the other three groups, the blood glucose of the diabetic heart and blood stasis syndrome group was higher than that of the other three groups, and the difference was significant (p < 0.01 or p < 0.05). (3) compared with the other three groups, the ratio of heart dampness weight to body weight and electrocardiogram in the diabetic heart and blood stasis group were significantly different from those of the other three groups (p < 0.01 or p < 0.05). (4) compared with the other three groups, the hemorheology index of the diabetes heart and blood stasis syndrome group was higher than that of the other three groups, and the difference was significant (p < 0.01 or p < 0.05). (5) compared with the other three groups, the morphological integral and the flow integral of the bulbar conjunctiva in the diabetic heart and blood stasis syndrome group showed significant difference (p < 0.01 or p < 0.05), but the pericardial integral was not abnormal (p > 0.05). Compared with the other three groups, the total cholesterol, triglyceride and high density lipoprotein were significantly increased in the diabetic heart and blood stasis group (P < 0.01 or p < 0.05). Under light microscope, there were inflammatory cell infiltration, cell necrosis and muscle fiber breakage in diabetic heart and blood stasis group. Under light microscope, aortic plaques were found in diabetic heart and blood stasis group. Conclusion\\\. 2. Based on the model of diabetes mellitus, the model of blood stasis syndrome was reconstituted, and the animal model of the combination of diabetes "disease" and "heart blood stasis" was established, which can be popularized.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R259;R-332

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