人FOXL2基因的克隆及其在大肠杆菌中的表达

发布时间：2018-03-05 02:09

本文选题：卵巢早衰　切入点：基因克隆　出处：《山东大学》2006年硕士论文　论文类型：学位论文

【摘要】：一、立题依据和目的卵巢早衰(premature ovarian failure POF)是指四十岁以前的女性有六个月以上的闭经并伴有雌激素水平降低和FSH水平大于40 IU/L的患者。卵巢早衰在一般人群中的发病率为1％，绝大多数的卵巢早衰患者为自发性。国内外学者研究发现这些患者的发病与遗传因素有密切关系。FOXL2基因是目前发现的第一个维持卵巢功能及与POF发病相关的常染色体基因。人FOXL2基因位于3q23，为单外显子基因，编码376氨基酸残基的FORHAED类的转录因子。免疫组织化学研究发现，FOXL2是核蛋白，无任何翻译后修饰，仅特异性的表达于哺乳动物的眼睑和卵巢组织。目前研究发现FORHAED家族的很多因子参与脊柱动物胚胎发育的调控，这个家族的某些因子的异常会导致遗传性疾病的发生。 FOXL2基因是目前研究发现的哺乳动物中最早的卵巢分化标志，，可能在卵巢早期的分化过程中起着重要的调控作用。同时由于它在出生后以及成人卵巢中的高度表达，显示FOXL2在卵泡的发育以及卵泡功能的维持中起着重要的作用。FOXL2在不同性别决定机制的脊柱动物中的高度保守并大量的表达显示是卵巢分化过程中最早的影响因子，有些学者认为这个基因是通过作用与TGFB家族的信号通路来影响卵巢的发育。为了更好的了解FOXL2在卵巢发育中的作用，就要了解它在分子调控途径中的作用机制。本实验以此为依据，采用基因工程的一系列实验方法，进行人FOXL2的基因克隆并在大肠杆菌中表达，从而获得FOXL2重组蛋白，为研究FOXL2在卵巢发育中的生物学功能奠定基础。二、方法和结果
[Abstract]:I. the basis and purpose of setting the subject. Premature ovarian failure (POF) is a group of women who have amenorrhea for more than six months before the age of 40 and are accompanied by a decrease in estrogen levels and FSH levels greater than 40 IU/L. The incidence of premature ovarian failure in the general population is 1. Premature ovarian failure patients are spontaneous. Domestic and foreign scholars have found that the pathogenesis of these patients is closely related to genetic factors. FOXL2 gene is the first autosomal gene to maintain ovarian function and be associated with POF pathogenesis. Human FOXL2 gene, located at 3q23, is a single exon gene and a transcription factor of FORHAED class encoding 376 amino acid residues. It is found that many factors of FORHAED family are involved in the regulation of embryonic development in spinal animals, and that the abnormality of some factors in this family may lead to the occurrence of genetic diseases. FOXL2 gene is the earliest marker of ovarian differentiation in mammals, which may play an important role in the early differentiation of ovary. At the same time, it is highly expressed in postnatal and adult ovaries. The results show that FOXL2 plays an important role in follicular development and follicular function maintenance. The highly conserved and high expression of FOXL2 in the sex determining mechanism of spinal cord shows that FOXL2 is the earliest influencing factor in the process of ovarian differentiation. Some researchers believe that the gene affects ovarian development by acting on the signaling pathway of the TGFB family, in order to better understand the role of FOXL2 in ovarian development. It is necessary to understand the mechanism of its action in the molecular regulatory pathway. Based on this, a series of experimental methods of genetic engineering were used to clone and express the human FOXL2 gene in E. coli, so as to obtain the recombinant FOXL2 protein. To lay a foundation for studying the biological function of FOXL2 in ovarian development. II. Methodology and results
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R711.7;R346

【共引文献】