辐照结合束缚应激致血虚肝郁大鼠证候模型的建立

发布时间：2018-03-07 08:13

本文选题：辐照　切入点：束缚应激　出处：《中华中医药杂志》2014年07期 　论文类型：期刊论文

【摘要】：目的:建立辐照结合束缚应激致血虚肝郁证大鼠模型。方法:SD雄性大鼠随机分为空白组、辐照组、束缚组、模型组,模型组采用大鼠单笼饲养、辐照结合束缚21d造模,检测各组大鼠体质量、外周血象并进行糖水实验、敞箱实验和跳台实验,测定血浆中促肾上腺皮质激素(ACTH)、皮质酮(CORT),下丘脑促肾上腺皮质激素释放激素(CRH)和大脑海马单胺类神经递质5-羟色胺(5-HT)和多巴胺(DA)等含量。结果:模型组大鼠生长缓慢,外周白细胞,红细胞、血红蛋白下降明显(P0.05),血浆中ACTH明显升高(P0.05),CORT和下丘脑CRH含量有升高趋势,脑内单胺类神经递质5-HT和DA含量明显降低(P0.05)。结论:从血象、行为学、下丘脑-垂体-肾上腺(HPA)轴和脑内单胺类神经递质等方面验证了辐照结合束缚应激造成的大鼠模型可作为中医血虚肝郁证的动物模型。
[Abstract]:Objective: to establish a rat model of blood deficiency and liver depression induced by irradiation combined with restraint stress. Methods: male SD rats were randomly divided into blank group, irradiation group, bound group, model group, model group and model group. The body mass, peripheral blood and sugar water, open box test and jumper test were measured in each group. The contents of adrenocorticotropic hormone (ACTH), cortisone, hypothalamic corticotropin releasing hormone (CRH) and hippocampal monoamine neurotransmitter 5-hydroxytryptamine (5-HT) and dopamine (DA) were measured. The contents of peripheral white blood cells, erythrocytes and hemoglobin decreased significantly (P0.05), the contents of P0.05Cort and CRH in hypothalamus increased significantly in plasma, and the contents of monoamine neurotransmitters 5-HT and DA in brain decreased P0.050.Conclusion: from hemogram, behaviorology, the contents of monoamine neurotransmitters 5-HT and DA are significantly decreased. The hypothalamus-pituitary-adrenal gland (HPA) axis and monoamine neurotransmitters in the brain proved that the rat model induced by irradiation combined with restraint stress could be used as an animal model of blood deficiency and liver depression in traditional Chinese medicine.
【作者单位】：北京中医药大学;
【基金】：国家自然科学基金(No.81173569) 北京中医药大学自主课题(No.2013-JYBZZ-XS-013)~~
【分类号】：R-332

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