离子造影剂增强Micro-CT扫描对大鼠关节软骨形态的定量分析

发布时间：2018-03-08 08:52

本文选题：Micro-CT　切入点：关节软骨　出处：《生物医学工程研究》2014年03期 　论文类型：期刊论文

【摘要】：利用Micro-CT技术的较高分辨精度,探索一种新的测量小动物关节软骨形态的方法,为进一步利用小动物模型开展关节软骨的损伤和退变等研究提供基础。选择不同浓度的造影剂及Micro-CT扫描条件,通过造影剂对比增强Micro-CT扫描大鼠膝关节,对大鼠股骨远端关节软骨进行手动分割、重建,得到软骨3D模型,利用重建得到的3D模型,对软骨的厚度和体积等形态学参数进行无创定量分析。根据造影剂、软骨和软骨下骨对X射线吸收的平均相对衰减值差异,确定30%的离子造影剂浓度为最佳。利用该浓度对4个不同的正常大鼠关节软骨进行扫描和重建,分析获得关节软骨的体积和厚度,并求得关节软骨体积和厚度的变异系数均方根分别为7.3%和6.1%。结果提示,利用造影剂增强Micro-CT扫描能够对软骨形态重要参数厚度、体积等进行定量评价。
[Abstract]:A new method for measuring the shape of articular cartilage in small animals was developed by using the high resolution precision of Micro-CT technique. In order to provide the basis for further research on the damage and degeneration of articular cartilage in small animal models, different concentrations of contrast agents and Micro-CT scanning conditions were selected, and contrast medium contrast enhanced Micro-CT scanning of the knee joint of rats. The articular cartilage of the distal femur of the rat was segmented manually and reconstructed, and the 3D model of cartilage was obtained. The thickness and volume of cartilage were analyzed by non-invasive quantitative analysis, according to the contrast medium, the thickness and volume of cartilage were quantitatively analyzed. The average relative attenuation value of X-ray absorption of cartilage and subchondral bone was different, and the best concentration of ion contrast agent 30% was determined, which was used to scan and reconstruct the articular cartilage of four different normal rats. The volume and thickness of articular cartilage were obtained, and the root mean square (RMS) of the volume and thickness of articular cartilage were 7.3% and 6.1, respectively. The results suggest that the thickness of cartilage morphology can be determined by contrast medium enhanced Micro-CT scan. The volume was evaluated quantitatively.
【作者单位】：北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室;
【基金】：国家自然科学基金资助项目(31271008,30800217) 中央高校基本科研业务费专项资金资助项目
【分类号】：R816.8;R-332

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