坐骨神经离断后tPA对脊髓神经细胞的影响

发布时间：2018-03-11 02:15

本文选题：坐骨神经　切入点：断后　出处：《汕头大学》2005年硕士论文　论文类型：学位论文

【摘要】：背景组织纤溶酶原激活物(tissue plasminogen actiVator,tPA) 是一种具有蛋白溶解活性的丝氨酸蛋白,它一直被临床用作脑栓塞急性期治疗的药物。但随着近年来研究的深入,发现tPA不仅具有溶栓的作用,而且它在神经元损伤时还可促使神经元死亡。另有一些证据表明 tPA有调节小胶质细胞活化和促进神经元凋亡的双重作用。由于小胶质细胞数量的增加和tPA浓度的高低相平行,tPA完全可被作为小胶质细胞增殖的一个指标。Ca~(2+)内流是引起tPA释放的主要因素,因此推测在坐骨神经离断后即在神经元轴突横断的情况下,神经元发生强烈的电活动,导致Ca~(2+)内流,先是受损的神经元释放少量的tPA来激活病变部位的小胶质细胞,这些活化的小胶质细胞再释放大量的tPA,一方面促使自身的增殖活化,另一方面当tPA超过神经元的致死浓度时,便引起了神经元的死亡。近年来在对tPA的研究中,人们大多是在中枢神经系统(central nervous system,CNS)损伤后来观察tPA的作用,这往往造成CNS中血管通透性的改变,血液中的tPA释放到CNS中,干扰实验结果.因此我们选择坐骨神经作为研究对象,在只造成坐骨神经损伤的前提下对tPA在脊髓中的作用进行初步研究。同时周围神经损伤后再生研究的重点已从局部的外科修复,发展到采用多因子多因素防止神经元胞体、神经纤维、效应器的变性,保护神经损伤后神经元,减少不可逆损伤的发生,综合有效地促进神经的再生。本课题的完成将为
[Abstract]:Background tissue plasminogen activator (plasminogen). Is a serine protein with proteolytic activity, which has been used clinically for acute cerebral embolism. But with the development of research in recent years, it is found that tPA not only has thrombolytic effect. It can also promote neuronal death in the event of neuronal injury. There is also some evidence that. TPA can regulate the activation of microglia and promote neuronal apoptosis. The increase of the number of cytoplasm cells parallel to the concentration of tPA can be used as microglia. An indicator of cell proliferation. Caan2) influx is the main factor causing tPA release. In the case of neuronal axon transection after sciatic nerve dissection, the neurons developed intense. Electrical activity, resulting in Ca~(2) inward flow, first damaged neurons release a small amount of tPA to activate the lesion. Microglia in the site, these activated microglia release a large amount of tPA, on the one hand, Promote self proliferation and activation, on the other hand, when tPA exceeds the lethal concentration of neurons, it causes. The death of neurons. In recent years, in the study of tPA, people are mostly in the central nervous system. To observe the role of tPA after central nervous damage. Causing changes in vascular permeability in CNS, releasing tPA from blood into CNS, interfering. So we chose the sciatic nerve as the object of study. The role of tPA in spinal cord was studied on the premise of injury. The focus of posttraumatic regeneration research has evolved from local surgical repair to multifactorial and multifactorial prevention. Degeneration of neuronal bodies, nerve fibers, and effectors, protection of neurons after nerve injury, reduction of neurons. The occurrence of less irreversible damage and the comprehensive and effective promotion of nerve regeneration. The completion of this project will be
【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R361

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