流行性感冒病毒于Vero细胞内繁殖的研究

发布时间：2018-03-15 05:04

本文选题：流行性感冒病毒　切入点：Vero细胞　出处：《吉林大学》2007年硕士论文　论文类型：学位论文

【摘要】： 流感是由甲(A)、乙(B)、丙(C)三型流感病毒分别引起的一种急性呼吸道传染病。甲、乙、丙不仅反映了病毒被发现的年代及前后顺序,更主要的是反映了对人类危害程度的顺序。其重要性在于可引起严重的发病率和死亡率,影响学校、公共服务机构和各单位的正常工作。超额死亡率也与流感有关,死亡者多为患有慢性疾病和年迈体衰的老年人。目前唯一可行的流感预防措施是疫苗接种。自1941年流感灭活疫苗在美国首次批准使用以来,流感疫苗已使用60多年。在此期间,广泛的经验已证明疫苗的安全性和效力。每年在流感流行季节前在流感的高危人群中进行预防接种是减轻流感危害的重要措施。疫苗接种不仅可以降低高危人群的发病率和死亡率,也可减轻症状,降低并发症的发生,降低继续传播的机率。目前研究应用于灭活流感疫苗生产的细胞系主要有MDCK、VERO、PER.C6三个细胞系。其中Vero传代细胞系解决了外源病毒和致癌性的问题,且易于大规模培养,被WHO推荐作为疫苗生产细胞基质之一,已成功开发出了狂犬病、脊髓灰质炎、流行性乙脑炎等疫苗。使用哺乳动物细胞培养技术替代鸡胚来生产流感疫苗将是大势所趋。基于上述情况,本文作者通过广泛的文献检索与反复试验,现已完成各(亚)型流感病毒于Vero细胞内的快速传代工艺,建立了流感病毒灭活疫苗(Vero细胞)生产用病毒种子批系统,解决了流感病毒于细胞内传代稳定性问题,为进一步研制流感病毒灭活疫苗(Vero细胞)打下了良好的基础。
[Abstract]:Influenza is an acute respiratory infectious disease caused by A, B, C) influenza viruses. A, B, C not only reflect the date and sequence of the virus being discovered. More importantly, it reflects the order of harm to human beings. Its importance lies in causing serious morbidity and mortality, affecting the normal work of schools, public services and units. Excess mortality is also associated with influenza. Most of the deaths are among the elderly with chronic illness and frailty. Vaccination is the only available influenza prevention measure. The flu vaccine has been in use for more than 60 years since 1941, when it was first approved for use in the United States. Extensive experience has proved the safety and effectiveness of vaccines. Vaccination every year before the influenza epidemic season in the influenza high-risk population is an important measure to reduce the risk of influenza. Vaccination can not only reduce the high-risk population. Morbidity and mortality, Can also reduce symptoms, reduce the incidence of complications, reduce the probability of further transmission. At present, the main cell lines used in the production of inactivated influenza vaccine are MDCK VEROPER.C6. Among them, Vero subculture cell line solves the problems of exogenous virus and carcinogenicity, and is easy to be cultured on a large scale. Rabies, poliomyelitis and Japanese encephalitis have been successfully developed as one of the cell matrix for vaccine production by WHO. It is a general trend to use mammalian cell culture technology to replace chicken embryo to produce influenza vaccine. In view of the above situation, the author has completed the rapid passage of various (subtype) influenza viruses in Vero cells through extensive literature retrieval and repeated experiments. A virus seed batch system was established for the production of inactivated influenza virus vaccine (Vero cells), which solved the problem of intracellular transmission stability of influenza virus and laid a good foundation for the further development of inactivated influenza virus vaccine Vero cells.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

【共引文献】