甲状旁腺腹腔移植模型的建立

发布时间：2018-03-16 10:21

本文选题：甲状旁腺　切入点：移植　出处：《浙江大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的:本实验旨在建立甲状旁腺(PTG)大网膜移植大鼠模型。方法:以成年雄性SD大鼠作为供体及受体,首先建立大鼠甲状旁腺功能低下受体模型,后切取供体双侧甲状旁腺植入受体大网膜上,观察移植前后血钙和血PTH变化情况,并于第1,2,4,6周切取受体大网膜行组织切片检查。结果:大网膜移植后第5天左右血钙即恢复至正常水平,平均2.1±0.44mmol/L,血PTH恢复至22.6±1.2ng/dL,持续4周左右,第5周血钙水平降至平均1.39±0.34mmol/L,血PTH亦降至8.5±2.0ng/dL(与移植前比P0.05)。组织切片提示相同结果。结论:在没有免疫抑制剂等干预因素下大鼠移植物在大网膜上能够存活大约4周,此方法能够建立较理想的PTG大网膜移植模型。
[Abstract]:Objective: to establish a rat model of parathyroid gland PTG (PTG) omentum transplantation. Methods: adult male SD rats were used as donors and receptors to establish the model of hypoparathyroidism receptor. The donor bilateral parathyroid glands were removed and implanted into the greater omentum. The changes of blood calcium and PTH were observed before and after transplantation. The recipient omentum was removed for histological examination at 6 weeks after transplantation. Results: on the 5th day after omentum transplantation, blood calcium returned to normal level (mean 2.1 卤0.44 mmol / L), blood PTH recovered to 22.6 卤1.2 ng / dL and lasted about 4 weeks. At the 5th week, the serum calcium level decreased to an average of 1.39 卤0.34 mmol / L, and the blood PTH also decreased to 8.5 卤2.0 ng / dL (compared with P0.05ng-1 / dL before transplantation). Tissue sections showed the same results. Conclusion: the grafts could survive on the omentum for about 4 weeks without the intervention of immunosuppressive agents. This method can establish an ideal model of PTG omentum transplantation.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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