雷公藤多甙致雌性小鼠肾虚生殖功能低下动物模型研究

发布时间：2018-03-16 16:35

本文选题：雷公藤多甙　切入点：昆明种小鼠　出处：《成都中医药大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：探讨雷公藤多甙造成雌性育龄期小鼠生殖功能低下的药理作用机理，建立雷公藤多甙致雌性小鼠生殖功能低下动物模型。方法：采用7～8周龄雌性成熟昆明种小鼠，通过阴道细胞学涂片筛选出有正常动情周期者，随机分为雷公藤多甙A、B、C、D、E5个剂量组及正常对照组，分别给予雷公藤多甙60mg／kg／d、40mg／kg／d、30mg／kg／d、20mg／kg／d、15mg／kg／d及生理盐水。连续每日观察给药后小鼠动情周期状态，根据动情周期变化情况推断给药后小鼠的生殖功能状态。分别在①各组部分小鼠开始出现动情周期延长，②各组小鼠动情周期均延长、不规则，③各组小鼠动情周期完全紊乱、甚至消失三个时段分批处死部分小鼠，以观察雷公藤多甙在不同时段对小鼠生殖功能的影响。采用放射免疫法检测血清E_2、P水平，组织称重法计算卵巢、子宫指数，病理切片观察各时段卵巢、子宫组织形态学变化，计数各级卵泡、黄体数，测量子宫内膜、肌层厚度。结果：给药5周时，各组部分小鼠出现动情周期延长；给药7周时，各组小鼠均出现动情周期延长、不规则；给药10周时，各组小鼠动情周期全部紊乱，部分动情周期消失，分别在5、7、10周时分批处死小鼠。结果显示：给药5周、7周时，雷公藤多甙不能降低E_2、P水平、卵巢指数和卵泡、黄体数，给药7周时A组子宫指数降低，内膜、肌层变薄。给药10周时，，A、B、C组子宫指数明显降低，子宫内膜、肌层明显变薄，卵巢成熟卵泡数及黄体数减少(P＜0.05或P＜0.01)；A、B组E_2水平降低(P＜0.05)，各组P水平和卵巢指数无明显变化。结论：1．雷公藤多甙给药10周时60mg／kg／d、40mg／kg／d及30mg／kg／d剂量均能在一定程度上损伤小鼠生殖功能，造成肾虚生殖功能低下状态。2．建议用雷公藤多甙30mg／kg／d给药10周以建立雌性小鼠肾虚生殖功能低下模型。
[Abstract]:Objective: to investigate the pharmacological mechanism of female reproductive dysfunction induced by tripterygium wilfordii polyglycosides, and to establish an animal model of female reproductive dysfunction induced by tripterygium wilfordii polyglycosides. Methods: female adult Kunming mice aged 7 to 8 weeks were selected by vaginal cytological smears. Those with normal estrous cycle were randomly divided into 5 dose group and normal control group of Tripterygium wilfordii. They were given 60 mg / kg / kg of Tripterygium wilfordii, 40 mg / kg / kg / d, 30 mg / kg / kg / d, 20 mg / kg / kg / d and 15 mg / kg / d of normal saline, respectively. The oestrous cycle of mice was observed continuously after administration of the drug daily. According to the changes of estrus cycle, the reproductive function of mice after administration was inferred. In order to observe the effect of tripterygium wilfordii polyglycosides on the reproductive function of mice, the serum E2P level was detected by radioimmunoassay, and the ovarian and uterine index was calculated by tissue weighing method. Histologic changes of ovary and uterus were observed by pathological section. Follicles and luteal bodies were counted and the thickness of endometrium and myometrium were measured. Results: at 5 weeks of administration, the estrus cycle of some mice in each group was prolonged; at 7 weeks, the estrus cycle was prolonged and irregular in all the mice of each group; at 10 weeks of administration, the estrus cycle of all the mice in each group was disordered, and part of the estrus cycle disappeared. Mice were killed in batches at 10 weeks after 5 weeks of administration. The results showed that tripterygium wilfordii polyglycosides could not decrease E2P level, ovarian index, follicle and corpus luteum at 5 weeks and 7 weeks after administration, and the uterine index and endometrium of group A were decreased at 7 weeks after administration of tripterygium wilfordii. After 10 weeks of administration, the uterine index, endometrium, myometrium and the number of mature follicles and luteal bodies decreased significantly (P < 0.05 or P < 0.01). Conclusion: 1. The dose of 60 mg / kg / kg / d and 30 mg / kg / d of tripterygium wilfordii can damage the reproductive function of mice to a certain extent after 10 weeks of administration of tripterygium wilfordii. It was suggested that 30 mg / kg / d of Tripterygium wilfordii polyglycosides should be given for 10 weeks in order to establish the model of low reproductive function in female mice with kidney deficiency.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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