家兔内囊出血模型的制作及体感诱发电位评价神经机能动态变化的意义

发布时间：2018-03-20 16:22

本文选题：内囊出血　切入点：体感诱发电位　出处：《天津医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的 1.建立家兔脑内囊出血的模型。 2.对内囊出血模型进行评价。 3.探讨内囊出血前后的体感诱发电位及自发脑电活动的变化,评价家兔神经机能的改变程度,为脑出血的诊断及临床治疗提供重要的参考依据。方法 1.家兔内囊出血模型的制备:麻醉家兔,固定于兔脑立体定位仪上。根据兔脑立体定位图谱,利用定位仪确定家兔内囊后肢位置,于定位点利用牙科钻施行颅骨打孔术,利用微量注射器在内囊位置注入家兔自体动脉血0.5ml,建立家兔内囊出血的动物模型。 2.家兔内囊出血前后体感诱发电位的计测:利用兔脑立体定位仪,确定家兔下肢在大脑皮层的感觉投射区的位置为诱发电位记录点的位置,于定位点实施颅骨打孔术,分离下肢胫后神经,将记录电极,参考电极,接地电极及刺激电极连接好,接通RM—6240B型生物信号采集系统,分别计测家兔内囊出血前后的诱发电位。 3.家兔内囊出血前后自发脑电活动的计测:确定家兔下肢在大脑皮层的感觉投射区的位置为家兔自发脑电活动记录点的位置,于定位点实施颅骨打孔术,将银球记录电极固定于记录点处,与脑膜紧密接触,连接好记录、参考、接地电极,接通RM—6240B型生物信号采集系统,分别计测家兔内囊出血前后的自发脑电活动。 4.家兔记录点与刺激点之间距离与体感诱发电位潜伏期关系的计测:利用皮尺测量家兔记录点与刺激点之间距离,利用分析软件对诱发电位P1、N2波的峰潜伏期进行测量。 5.家兔内囊出血后脑组织大体病理标本的制备及出血前后脑组织病理形态学
[Abstract]:Purpose. 1. To establish the model of intracerebral capsule hemorrhage in rabbits. 2. To evaluate the model of internal capsule hemorrhage. 3. To investigate the changes of somatosensory evoked potential (SEP) and spontaneous EEG activity before and after intracerebral hemorrhage, and to evaluate the degree of changes in nerve function in rabbits, and to provide an important reference for the diagnosis and clinical treatment of intracerebral hemorrhage. Method. 1. Preparation of rabbit internal capsule hemorrhage model: anesthetized rabbits were fixed on the rabbit brain stereotactic locator. According to the stereotaxic map of rabbit brain, the position of the posterior limb of the rabbit internal capsule was determined by the locator, and the skull perforation was performed by dental drill at the location point. The rabbit model of intracystic hemorrhage was established by injecting rabbit autologous arterial blood (0.5 ml) into the internal capsule with a micro syringe. 2. Measurement of somatosensory evoked potential (SEP) before and after intracapsular hemorrhage in rabbits: the location of the sensory projection area of the rabbit lower extremity in the cerebral cortex was determined as the recording point of the evoked potential by using the stereotactic locator of the rabbit brain, and the skull perforation was performed at the location point. The posterior tibial nerve of the lower extremity was separated and the recording electrode, reference electrode, grounding electrode and stimulation electrode were connected together, and the RM-6240B biological signal acquisition system was connected to measure the evoked potentials before and after the internal capsule hemorrhage in rabbits. 3. Measurement of spontaneous EEG activity before and after intracapsular hemorrhage in rabbits: the location of the sensory projection area of the lower extremity in the cerebral cortex was determined as the recording point of the spontaneous EEG activity of the rabbit, and the skull perforation was performed at the location point. The silver ball recording electrode was fixed at the recording point, in close contact with the meninges, connected with the recording, referred to, grounded electrode, and connected to the RM-6240B type biological signal acquisition system. The spontaneous EEG activity before and after intracystic hemorrhage in rabbits was measured respectively. 4.The relationship between the distance between the recording point and the stimulation point and the latency of somatosensory evoked potential (SEP) was measured. The distance between the recording point and the stimulation point was measured by using the scale, and the peak latency of the evoked potential P1N _ 2 wave was measured by the software of analysis. 5. Preparation of gross pathological specimen of brain tissue after intracapsular hemorrhage in rabbits and pathological morphology of brain tissue before and after hemorrhage
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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